Combined electrophoresis-electrospray interface and method
Abstract
A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.
- Inventors:
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176693
- Patent Number(s):
- RE34757
- Application Number:
- 07/803,145
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Smith, Richard D., Udseth, Harold R., and Olivares, Jose A. Combined electrophoresis-electrospray interface and method. United States: N. p., 1994.
Web.
Smith, Richard D., Udseth, Harold R., & Olivares, Jose A. Combined electrophoresis-electrospray interface and method. United States.
Smith, Richard D., Udseth, Harold R., and Olivares, Jose A. Tue .
"Combined electrophoresis-electrospray interface and method". United States. https://www.osti.gov/servlets/purl/1176693.
@article{osti_1176693,
title = {Combined electrophoresis-electrospray interface and method},
author = {Smith, Richard D. and Udseth, Harold R. and Olivares, Jose A.},
abstractNote = {A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 18 00:00:00 EDT 1994},
month = {Tue Oct 18 00:00:00 EDT 1994}
}