Combined electrophoresis-electrospray interface and method
Abstract
A system and method for analyzing molecular constituents of a composition sample include: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5--100 kVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g.,{+-}2--8 kVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit. 21 figs.
- Inventors:
- Issue Date:
- Research Org.:
- Battelle Memorial Institute, Columbus, OH (United States)
- OSTI Identifier:
- 27740
- Patent Number(s):
- 34757
- Application Number:
- PAN: 7-803,145
- Assignee:
- Battelle Memorial Inst., Richland, WA (United States)
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Other Information: DN: Reissue of US Pat. No. 4,885,076, which was issued Dec. 5, 1989; PBD: 18 Oct 1994
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 40 CHEMISTRY; MIXTURES; ELECTROPHORESIS; ATOMIZATION; SAMPLE PREPARATION; ELECTRIC FIELDS; ELECTROLYTIC CELLS; ELECTRODES
Citation Formats
Smith, R D, Udseth, H R, and Olivares, J A. Combined electrophoresis-electrospray interface and method. United States: N. p., 1994.
Web.
Smith, R D, Udseth, H R, & Olivares, J A. Combined electrophoresis-electrospray interface and method. United States.
Smith, R D, Udseth, H R, and Olivares, J A. Tue .
"Combined electrophoresis-electrospray interface and method". United States.
@article{osti_27740,
title = {Combined electrophoresis-electrospray interface and method},
author = {Smith, R D and Udseth, H R and Olivares, J A},
abstractNote = {A system and method for analyzing molecular constituents of a composition sample include: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5--100 kVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g.,{+-}2--8 kVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit. 21 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {10}
}