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Title: Combined electrophoresis-electrospray interface and method

Abstract

A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.

Inventors:
 [1];  [1];  [2]
  1. Richland, WA
  2. Los Alamos, NM
Issue Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA Battelle Memorial Institute
OSTI Identifier:
875295
Patent Number(s):
RE34757
Assignee:
Battelle Memorial Institute (Richland, WA)
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
combined; electrophoresis-electrospray; interface; method; analyzing; molecular; constituents; composition; sample; forming; solution; separating; capillary; electrophoresis; eluent; longitudinally; separated; according; relative; electrophoretic; mobilities; electrospraying; form; charged; spray; temporal; distribution; detecting; collecting; accordance; high-voltage; 5-100; kvdc; applied; applying; voltage; 2-8; exit; cathode; spaced; front; complete; electrical; circuit; formed; conductor; directly; contacts; conduction; sheath; electrode; discharged; annular; flow; temporal distribution; molecular constituents; sheath flow; capillary electrophoresis; electrical circuit; capillary exit; relative electrophoretic; separated according; separated constituents; charged spray; combined electrophoresis-electrospray; composition sample; complete electrical; cathode spaced; analyzing molecular; electrophoresis-electrospray interface; electrophoretic mobilities; longitudinally separated; constituents longitudinally; directly contacts; MIXTURES; ATOMIZATION; SAMPLE PREPARATION; ELECTRIC FIELDS; ELECTROLYTIC CELLS; ELECTRODES; /204/250/

Citation Formats

Smith, Richard D., Udseth, Harold R., and Olivares, Jose A. Combined electrophoresis-electrospray interface and method. United States: N. p., 1994. Web.
Smith, Richard D., Udseth, Harold R., & Olivares, Jose A. Combined electrophoresis-electrospray interface and method. United States.
Smith, Richard D., Udseth, Harold R., and Olivares, Jose A. Tue . "Combined electrophoresis-electrospray interface and method". United States. https://www.osti.gov/servlets/purl/875295.
@article{osti_875295,
title = {Combined electrophoresis-electrospray interface and method},
author = {Smith, Richard D. and Udseth, Harold R. and Olivares, Jose A.},
abstractNote = {A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {10}
}

Works referenced in this record:

Capillary zone electrophoresis
journal, October 1983


Electrospray mass spectroscopy of macromolecules: Application of an ion-drift spectrometer
journal, June 1984


Electrospray interface for liquid chromatographs and mass spectrometers
journal, March 1985


Electrospray mass spectroscopy of macromolecule degradation in the electrospray
journal, May 1975


Negative ion production with the electrospray ion source
journal, September 1984


Electrospray ion source. Another variation on the free-jet theme
journal, September 1984