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Title: Combined electrophoresis-electrospray interface and method

A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.
Inventors:
 [1];  [1];  [2]
  1. Richland, WA
  2. Los Alamos, NM
Issue Date:
OSTI Identifier:
875295
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
Patent Number(s):
US RE34757
Contract Number:
AC06-76RL01830
Research Org:
Pacific Northwest National Laboratory (PNNL), Richland, WA Battelle Memorial Institute
Country of Publication:
United States
Language:
English
Subject:
combined; electrophoresis-electrospray; interface; method; analyzing; molecular; constituents; composition; sample; forming; solution; separating; capillary; electrophoresis; eluent; longitudinally; separated; according; relative; electrophoretic; mobilities; electrospraying; form; charged; spray; temporal; distribution; detecting; collecting; accordance; high-voltage; 5-100; kvdc; applied; applying; voltage; 2-8; exit; cathode; spaced; front; complete; electrical; circuit; formed; conductor; directly; contacts; conduction; sheath; electrode; discharged; annular; flow; temporal distribution; molecular constituents; sheath flow; capillary electrophoresis; electrical circuit; capillary exit; relative electrophoretic; separated according; separated constituents; charged spray; combined electrophoresis-electrospray; composition sample; complete electrical; cathode spaced; analyzing molecular; electrophoresis-electrospray interface; electrophoretic mobilities; longitudinally separated; constituents longitudinally; directly contacts; MIXTURES; ATOMIZATION; SAMPLE PREPARATION; ELECTRIC FIELDS; ELECTROLYTIC CELLS; ELECTRODES; /204/250/