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Title: Constructs and methods for genome editing and genetic engineering of fungi and protists

Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.
Inventors:
;
Issue Date:
OSTI Identifier:
1418981
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) CHO
Patent Number(s):
9,879,270
Application Number:
14/826,566
Contract Number:
FC02-07ER64494
Resource Relation:
Patent File Date: 2015 Aug 14
Research Org:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
journal, July 2013

Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae
book, January 2013