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Title: Constructs and methods for genome editing and genetic engineering of fungi and protists

Abstract

Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

Inventors:
;
Issue Date:
Research Org.:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1418981
Patent Number(s):
9879270
Application Number:
14/826,566
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Aug 14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Hittinger, Christopher Todd, and Alexander, William Gerald. Constructs and methods for genome editing and genetic engineering of fungi and protists. United States: N. p., 2018. Web.
Hittinger, Christopher Todd, & Alexander, William Gerald. Constructs and methods for genome editing and genetic engineering of fungi and protists. United States.
Hittinger, Christopher Todd, and Alexander, William Gerald. Tue . "Constructs and methods for genome editing and genetic engineering of fungi and protists". United States. https://www.osti.gov/servlets/purl/1418981.
@article{osti_1418981,
title = {Constructs and methods for genome editing and genetic engineering of fungi and protists},
author = {Hittinger, Christopher Todd and Alexander, William Gerald},
abstractNote = {Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {1}
}

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Works referenced in this record:

High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces
journal, September 2014


Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
journal, March 2013


ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
journal, July 2013


The CRISPR Craze
journal, August 2013


In vivo site-directed mutagenesis using oligonucleotides
journal, August 2001


Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae
book, January 2013