Constructs and methods for genome editing and genetic engineering of fungi and protists
Abstract
Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.
- Inventors:
- Issue Date:
- Research Org.:
- Wisconsin Alumni Research Foundation, Madison, WI (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1418981
- Patent Number(s):
- 9879270
- Application Number:
- 14/826,566
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FC02-07ER64494
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2015 Aug 14
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Hittinger, Christopher Todd, and Alexander, William Gerald. Constructs and methods for genome editing and genetic engineering of fungi and protists. United States: N. p., 2018.
Web.
Hittinger, Christopher Todd, & Alexander, William Gerald. Constructs and methods for genome editing and genetic engineering of fungi and protists. United States.
Hittinger, Christopher Todd, and Alexander, William Gerald. Tue .
"Constructs and methods for genome editing and genetic engineering of fungi and protists". United States. https://www.osti.gov/servlets/purl/1418981.
@article{osti_1418981,
title = {Constructs and methods for genome editing and genetic engineering of fungi and protists},
author = {Hittinger, Christopher Todd and Alexander, William Gerald},
abstractNote = {Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 30 00:00:00 EST 2018},
month = {Tue Jan 30 00:00:00 EST 2018}
}
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