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Title: Constructs and methods for genome editing and genetic engineering of fungi and protists

Abstract

Provided herein are constructs for genome editing or genetic engineering its fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

Inventors:
;
Issue Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1771742
Patent Number(s):
10870858
Application Number:
15/878,698
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 01/24/2018
Country of Publication:
United States
Language:
English

Citation Formats

Hittinger, Christopher Todd, and Alexander, William Gerald. Constructs and methods for genome editing and genetic engineering of fungi and protists. United States: N. p., 2020. Web.
Hittinger, Christopher Todd, & Alexander, William Gerald. Constructs and methods for genome editing and genetic engineering of fungi and protists. United States.
Hittinger, Christopher Todd, and Alexander, William Gerald. Tue . "Constructs and methods for genome editing and genetic engineering of fungi and protists". United States. https://www.osti.gov/servlets/purl/1771742.
@article{osti_1771742,
title = {Constructs and methods for genome editing and genetic engineering of fungi and protists},
author = {Hittinger, Christopher Todd and Alexander, William Gerald},
abstractNote = {Provided herein are constructs for genome editing or genetic engineering its fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {12}
}

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