Constructs and methods for genome editing and genetic engineering of fungi and protists

Hittinger, Christopher Todd; Alexander, William Gerald

Title: Constructs and methods for genome editing and genetic engineering of fungi and protists

Patent · Tue Dec 22 00:00:00 EST 2020

OSTI ID:1771742

Hittinger, Christopher Todd; Alexander, William Gerald

Provided herein are constructs for genome editing or genetic engineering its fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC02-07ER64494

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Number(s):: 10,870,858

Application Number:: 15/878,698

OSTI ID:: 1771742

Resource Relation:: Patent File Date: 01/24/2018

Country of Publication:: United States

Language:: English

References (22)

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