Assembly of nucleic acid sequences in emulsions
Abstract
Methods and compositions for synthesizing nucleic acid sequences in an emulsion are provided.
- Inventors:
- Issue Date:
- Research Org.:
- Harvard Univ., Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1502836
- Patent Number(s):
- 10202628
- Application Number:
- 14/379,005
- Assignee:
- President and Fellows of Harvard College (Cambridge, MA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG02-02ER63445
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2013 Feb 14
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Church, George M., Terry, Richard C., Kosuri, Sriram, and Zhang, Di. Assembly of nucleic acid sequences in emulsions. United States: N. p., 2019.
Web.
Church, George M., Terry, Richard C., Kosuri, Sriram, & Zhang, Di. Assembly of nucleic acid sequences in emulsions. United States.
Church, George M., Terry, Richard C., Kosuri, Sriram, and Zhang, Di. Tue .
"Assembly of nucleic acid sequences in emulsions". United States. https://www.osti.gov/servlets/purl/1502836.
@article{osti_1502836,
title = {Assembly of nucleic acid sequences in emulsions},
author = {Church, George M. and Terry, Richard C. and Kosuri, Sriram and Zhang, Di},
abstractNote = {Methods and compositions for synthesizing nucleic acid sequences in an emulsion are provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 12 00:00:00 EST 2019},
month = {Tue Feb 12 00:00:00 EST 2019}
}
Works referenced in this record:
High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides
journal, August 2010
- Borovkov, Alex Y.; Loskutov, Andrey V.; Robida, Mark D.
- Nucleic Acids Research, Vol. 38, Issue 19
Methods and devices for high fidelity polynucleotide synthesis
patent, August 2014
- Jacobson, Joseph M.; Church, George M.; Chu, Larry Li-Yang
- US Patent Document 8,808,986
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips
journal, November 2010
- Kosuri, Sriram; Eroshenko, Nikolai; LeProust, Emily M.
- Nature Biotechnology, Vol. 28, Issue 12, p. 1295-1299
Next-generation DNA sequencing
journal, October 2008
- Shendure, Jay; Ji, Hanlee
- Nature Biotechnology, Vol. 26, Issue 10