Chip-based sequencing nucleic acids

Beer, Neil Reginald

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Title: Chip-based sequencing nucleic acids

Abstract

A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.

Inventors:: Beer, Neil Reginald

Issue Date:: Tue Aug 26 00:00:00 EDT 2014

Research Org.:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1151768

Patent Number(s):: 8815576

Application Number:: 11/965,585

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B01L2200/0668 - Trapping microscopic beads
B01L2200/0673 - Handling of plugs of fluid surrounded by immiscible fluid
B01L2300/0816 - Cards, e.g. flat sample carriers usually with flow in two horizontal directions
B01L2300/1827 - using resistive heater
B01L2300/1861 - using radiation
B01L2400/043 - magnetic forces
B01L2400/0487 - fluid pressure, pneumatics
B01L3/502761 - {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
B01L3/502784 - {specially adapted for droplet or plug flow, e.g. digital microfluidics
B01L7/52 - {with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6834 - Enzymatic or biochemical coupling of nucleic acids to a solid phase
C12Q1/6869 - Methods for sequencing
C12Q2563/143 - Magnetism, e.g. magnetic label
C12Q2563/155 - Particles of a defined size, e.g. nanoparticles
C12Q2565/629 - being a microfluidic device

Show less

DOE Contract Number:: AC52-07NA27344

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Beer, Neil Reginald. Chip-based sequencing nucleic acids.  United States: N. p., 2014. 
        Web.

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                    Beer, Neil Reginald. Chip-based sequencing nucleic acids.  United States.

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                    Beer, Neil Reginald. Tue .  
        "Chip-based sequencing nucleic acids".  United States.  https://www.osti.gov/servlets/purl/1151768.

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@article{osti_1151768,

  title        = {Chip-based sequencing nucleic acids},

  author       = {Beer, Neil Reginald},

  abstractNote = {A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Aug 26 00:00:00 EDT 2014},

  month        = {Tue Aug 26 00:00:00 EDT 2014}

}

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Similar Records

Title: Chip-based sequencing nucleic acids

Abstract

Citation Formats

Mesoscale polynucleotide amplification device and method patent, March 1996

Methods for sorting polynucleotides using oligonucleotide tags patent, February 1997

Solid supports for nucleic acid hybridization assays patent, September 1997

DNA purification and isolation using magnetic particles patent, January 1998

Wax spatula apparatus patent-application, February 2002

Polyadditions in aqueous and non-aqueous miniemulsions patent-application, March 2002

Microfluidic method for nucleic acid purification and processing patent-application, August 2002

Chip-based isothermal amplification devices and methods patent-application, November 2002

Method of DNA sequencing using cleavable tags patent-application, January 2003

Apparatus and method for sequencing a nucleic acid patent-application, May 2003

Nucleic acid sequencing using microsphere arrays patent-application, June 2003

Method and apparatus for temperature gradient microfluidics patent-application, January 2004

Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control patent-application, June 2004

Manipulation of microparticles in microfluidic systems patent-application, May 2004

Solid phase technique for selectively isolating nucleic acids patent-application, October 2004

Single molecule amplification and detection of DNA length patent-application, February 2005

Method and device for biochemical detection and analysis of subcellular compartments from a single cell patent-application, March 2005

Method and device for introducing a sample into an electrophoretic apparatus patent-application, April 2005

Bioconjugated nanostructures, methods of fabrication thereof, and methods of use thereof patent-application, June 2005

Nucleic acid sequencing using microsphere arrays patent-application, November 2005

Thermal reaction device and method for using the same patent-application, November 2005

Microfabricated integrated DNA analysis system patent-application, December 2005

Method for isolation of independent, parallel chemical micro-reactions using a porous filter patent-application, January 2006

Device for immobilizing chemical and biochemical species and methods of using same patent-application, August 2006

Droplet-based surface modification and washing patent-application, October 2007

Microfluidic devices and methods of use thereof patent-application, January 2008

Mesoscale polynucleotide amplification device and method
patent, March 1996

Methods for sorting polynucleotides using oligonucleotide tags
patent, February 1997

Solid supports for nucleic acid hybridization assays
patent, September 1997

DNA purification and isolation using magnetic particles
patent, January 1998

Wax spatula apparatus
patent-application, February 2002

Polyadditions in aqueous and non-aqueous miniemulsions
patent-application, March 2002

Microfluidic method for nucleic acid purification and processing
patent-application, August 2002

Chip-based isothermal amplification devices and methods
patent-application, November 2002

Method of DNA sequencing using cleavable tags
patent-application, January 2003

Apparatus and method for sequencing a nucleic acid
patent-application, May 2003

Nucleic acid sequencing using microsphere arrays
patent-application, June 2003

Method and apparatus for temperature gradient microfluidics
patent-application, January 2004

Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
patent-application, June 2004

Manipulation of microparticles in microfluidic systems
patent-application, May 2004

Solid phase technique for selectively isolating nucleic acids
patent-application, October 2004

Single molecule amplification and detection of DNA length
patent-application, February 2005

Method and device for biochemical detection and analysis of subcellular compartments from a single cell
patent-application, March 2005

Method and device for introducing a sample into an electrophoretic apparatus
patent-application, April 2005

Bioconjugated nanostructures, methods of fabrication thereof, and methods of use thereof
patent-application, June 2005

Nucleic acid sequencing using microsphere arrays
patent-application, November 2005

Thermal reaction device and method for using the same
patent-application, November 2005

Microfabricated integrated DNA analysis system
patent-application, December 2005

Method for isolation of independent, parallel chemical micro-reactions using a porous filter
patent-application, January 2006

Device for immobilizing chemical and biochemical species and methods of using same
patent-application, August 2006

Droplet-based surface modification and washing
patent-application, October 2007

Microfluidic devices and methods of use thereof
patent-application, January 2008