skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Kit for detecting nucleic acid sequences using competitive hybridization probes

Abstract

A kit is provided for detecting a target nucleic acid sequence in a sample, the kit comprising: a first hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the first hybridization probe including a first complexing agent for forming a binding pair with a second complexing agent; and a second hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the first hybridization probe does not selectively hybridize, the second hybridization probe including a detectable marker; a third hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the third hybridization probe including the same detectable marker as the second hybridization probe; and a fourth hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the third hybridization probe does not selectively hybridize, the fourth hybridization probe including the first complexing agent for forming a binding pair with the second complexing agent; whereinmore » the first and second hybridization probes are capable of simultaneously hybridizing to the target sequence and the third and fourth hybridization probes are capable of simultaneously hybridizing to the target sequence, the detectable marker is not present on the first or fourth hybridization probes and the first, second, third, and fourth hybridization probes each include a competitive nucleic acid sequence which is sufficiently complementary to a third portion of the target sequence that the competitive sequences of the first, second, third, and fourth hybridization probes compete with each other to hybridize to the third portion of the target sequence.« less

Inventors:
 [1];  [2];  [3]
  1. (San Ramon, CA)
  2. (Tracy, CA)
  3. (Walnut Creek, CA)
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
873914
Patent Number(s):
6270972
Assignee:
Regents of University of California (Berkeley, CA) LLNL
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
kit; detecting; nucleic; acid; sequences; competitive; hybridization; probes; provided; target; sequence; sample; comprising; probe; sufficiently; complementary; selectively; hybridize; portion; including; complexing; agent; forming; binding; pair; detectable; marker; third; fourth; capable; simultaneously; hybridizing; compete; detecting nucleic; detectable marker; probe including; acid sequence; nucleic acid; acid sequences; target sequence; target nucleic; complexing agent; hybridization probe; hybridization probes; sufficiently complementary; binding pair; target nuclei; competitive hybridization; /435/999/

Citation Formats

Lucas, Joe N., Straume, Tore, and Bogen, Kenneth T. Kit for detecting nucleic acid sequences using competitive hybridization probes. United States: N. p., 2001. Web.
Lucas, Joe N., Straume, Tore, & Bogen, Kenneth T. Kit for detecting nucleic acid sequences using competitive hybridization probes. United States.
Lucas, Joe N., Straume, Tore, and Bogen, Kenneth T. Mon . "Kit for detecting nucleic acid sequences using competitive hybridization probes". United States. https://www.osti.gov/servlets/purl/873914.
@article{osti_873914,
title = {Kit for detecting nucleic acid sequences using competitive hybridization probes},
author = {Lucas, Joe N. and Straume, Tore and Bogen, Kenneth T.},
abstractNote = {A kit is provided for detecting a target nucleic acid sequence in a sample, the kit comprising: a first hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the first hybridization probe including a first complexing agent for forming a binding pair with a second complexing agent; and a second hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the first hybridization probe does not selectively hybridize, the second hybridization probe including a detectable marker; a third hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a first portion of the target sequence, the third hybridization probe including the same detectable marker as the second hybridization probe; and a fourth hybridization probe which includes a nucleic acid sequence that is sufficiently complementary to selectively hybridize to a second portion of the target sequence to which the third hybridization probe does not selectively hybridize, the fourth hybridization probe including the first complexing agent for forming a binding pair with the second complexing agent; wherein the first and second hybridization probes are capable of simultaneously hybridizing to the target sequence and the third and fourth hybridization probes are capable of simultaneously hybridizing to the target sequence, the detectable marker is not present on the first or fourth hybridization probes and the first, second, third, and fourth hybridization probes each include a competitive nucleic acid sequence which is sufficiently complementary to a third portion of the target sequence that the competitive sequences of the first, second, third, and fourth hybridization probes compete with each other to hybridize to the third portion of the target sequence.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

Patent:

Save / Share: