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Title: Solid phase sequencing of double-stranded nucleic acids

Abstract

This invention relates to methods for detecting and sequencing of target double-stranded nucleic acid sequences, to nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probe comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include nucleic acids in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated determination of molecular weights and identification of the target sequence.

Inventors:
 [1];  [2];  [3];  [2]
  1. (Waltham, MA)
  2. (Boston, MA)
  3. (Concord, MA)
Issue Date:
Research Org.:
Boston University
OSTI Identifier:
874666
Patent Number(s):
6436635
Assignee:
Boston University (Boston, MA); Sequenom, Inc. (San Diego, CA) CHO
DOE Contract Number:  
FG02-93ER61609
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
solid; phase; sequencing; double-stranded; nucleic; acids; relates; methods; detecting; target; acid; sequences; probes; arrays; useful; kits; systems; contain; involve; hybridizing; represent; complementary; homologous; array; probe; single-stranded; portion; optional; variable; sequence; molecular; weights; hybridized; set; determined; mass; spectroscopy; fragments; biological; samples; patient; biopsies; environmental; fixed; support; hybridization; chip; facilitate; automated; determination; identification; nucleic acid; molecular weight; acid sequence; solid support; acid probe; stranded nucleic; /435/999/

Citation Formats

Fu, Dong-Jing, Cantor, Charles R., Koster, Hubert, and Smith, Cassandra L. Solid phase sequencing of double-stranded nucleic acids. United States: N. p., 2002. Web.
Fu, Dong-Jing, Cantor, Charles R., Koster, Hubert, & Smith, Cassandra L. Solid phase sequencing of double-stranded nucleic acids. United States.
Fu, Dong-Jing, Cantor, Charles R., Koster, Hubert, and Smith, Cassandra L. Tue . "Solid phase sequencing of double-stranded nucleic acids". United States. https://www.osti.gov/servlets/purl/874666.
@article{osti_874666,
title = {Solid phase sequencing of double-stranded nucleic acids},
author = {Fu, Dong-Jing and Cantor, Charles R. and Koster, Hubert and Smith, Cassandra L.},
abstractNote = {This invention relates to methods for detecting and sequencing of target double-stranded nucleic acid sequences, to nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probe comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include nucleic acids in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated determination of molecular weights and identification of the target sequence.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {1}
}

Patent:

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