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Title: Chip-based sequencing nucleic acids

Patent ·
OSTI ID:1151768

A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC52-07NA27344
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Number(s):
8,815,576
Application Number:
11/965,585
OSTI ID:
1151768
Country of Publication:
United States
Language:
English

References (26)

Mesoscale polynucleotide amplification device and method patent March 1996
Methods for sorting polynucleotides using oligonucleotide tags patent February 1997
Solid supports for nucleic acid hybridization assays patent September 1997
DNA purification and isolation using magnetic particles patent January 1998
Wax spatula apparatus patent-application February 2002
Polyadditions in aqueous and non-aqueous miniemulsions patent-application March 2002
Microfluidic method for nucleic acid purification and processing patent-application August 2002
Chip-based isothermal amplification devices and methods patent-application November 2002
Method of DNA sequencing using cleavable tags patent-application January 2003
Apparatus and method for sequencing a nucleic acid patent-application May 2003
Nucleic acid sequencing using microsphere arrays patent-application June 2003
Method and apparatus for temperature gradient microfluidics patent-application January 2004
Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control patent-application June 2004
Manipulation of microparticles in microfluidic systems patent-application May 2004
Solid phase technique for selectively isolating nucleic acids patent-application October 2004
Single molecule amplification and detection of DNA length patent-application February 2005
Method and device for biochemical detection and analysis of subcellular compartments from a single cell patent-application March 2005
Method and device for introducing a sample into an electrophoretic apparatus patent-application April 2005
Bioconjugated nanostructures, methods of fabrication thereof, and methods of use thereof patent-application June 2005
Nucleic acid sequencing using microsphere arrays patent-application November 2005
Thermal reaction device and method for using the same patent-application November 2005
Microfabricated integrated DNA analysis system patent-application December 2005
Method for isolation of independent, parallel chemical micro-reactions using a porous filter patent-application January 2006
Device for immobilizing chemical and biochemical species and methods of using same patent-application August 2006
Droplet-based surface modification and washing patent-application October 2007
Microfluidic devices and methods of use thereof patent-application January 2008

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59 BASIC BIOLOGICAL SCIENCES