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Title: Modulated excitation fluorescence analysis

Abstract

Fluorescence, spectroscopy is used to analyze small numbers of molecules that are present in a relatively small detection volume or zone. Information regarding physical and chemical properties of these molecules is determined by rapidly modulating the wavelength, intensity and/or polarization of laser energy to excite fluorophores that are attached either to the molecule of interest or a molecule that interacts with the molecule of interest. The emission profile of the fluorophores is used to determine useful information about the labeled and/or non-labeled molecules including molecular interactions between the molecules.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531572
Patent Number(s):
7,456,954
Application Number:
10/561,448
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
FG03-02ER63339
Resource Type:
Patent
Resource Relation:
Patent File Date: 2004-06-17
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; 42 ENGINEERING

Citation Formats

Weiss, Shimon, Kapanidis, Achillefs, Laurence, Ted A., and Lee, Nam K. Modulated excitation fluorescence analysis. United States: N. p., 2008. Web.
Weiss, Shimon, Kapanidis, Achillefs, Laurence, Ted A., & Lee, Nam K. Modulated excitation fluorescence analysis. United States.
Weiss, Shimon, Kapanidis, Achillefs, Laurence, Ted A., and Lee, Nam K. Tue . "Modulated excitation fluorescence analysis". United States. https://www.osti.gov/servlets/purl/1531572.
@article{osti_1531572,
title = {Modulated excitation fluorescence analysis},
author = {Weiss, Shimon and Kapanidis, Achillefs and Laurence, Ted A. and Lee, Nam K.},
abstractNote = {Fluorescence, spectroscopy is used to analyze small numbers of molecules that are present in a relatively small detection volume or zone. Information regarding physical and chemical properties of these molecules is determined by rapidly modulating the wavelength, intensity and/or polarization of laser energy to excite fluorophores that are attached either to the molecule of interest or a molecule that interacts with the molecule of interest. The emission profile of the fluorophores is used to determine useful information about the labeled and/or non-labeled molecules including molecular interactions between the molecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {11}
}

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System for distinguishing fluorescent molecule groups by time resolved fluorescence measurement
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Single-channel multicolored correlation analysis
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