Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer
Abstract
A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.
- Inventors:
-
- Clayton, MO
- St. Louis, MO
- Issue Date:
- Research Org.:
- Univ. of Washington, Seattle, WA (United States)
- OSTI Identifier:
- 872487
- Patent Number(s):
- 5945283
- Assignee:
- Washington University (St. Louis, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG06-94ER61909
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- methods; kits; nucleic; acid; analysis; fluorescence; resonance; energy; transfer; method; detecting; presence; target; nucleotide; sequence; nucleotides; disclosed; comprised; forming; oligonucleotide; labeled; fluorophores; site; doubly; formed; addition; singly; dideoxynucleoside; triphosphate; polynucleotide; ligation; polynucleotides; detection; denaturation; indicates; provided; particularly; applicable; genotyping; resonance energy; energy transfer; particularly applicable; nucleic acid; target nucleotide; target site; acid target; nucleoside triphosphate; /435/436/
Citation Formats
Kwok, Pui-Yan, and Chen, Xiangning. Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer. United States: N. p., 1999.
Web.
Kwok, Pui-Yan, & Chen, Xiangning. Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer. United States.
Kwok, Pui-Yan, and Chen, Xiangning. Fri .
"Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer". United States. https://www.osti.gov/servlets/purl/872487.
@article{osti_872487,
title = {Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer},
author = {Kwok, Pui-Yan and Chen, Xiangning},
abstractNote = {A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}
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Works referenced in this record:
Allelic discrimination by nick-translation PCR with fluorgenic probes
journal, August 1993
- Lee, Linda G.; Connell, Charles R.; Bloch, Will
- Nucleic Acids Research, Vol. 21, Issue 16, p. 3761-3766
Real time quantitative PCR.
journal, October 1996
- Heid, C. A.; Stevens, J.; Livak, K. J.
- Genome Research, Vol. 6, Issue 10, p. 986-994
A ligase-mediated gene detection technique
journal, August 1988
- Landegren, U.; Kaiser, R.; Sanders, J.
- Science, Vol. 241, Issue 4869
A novel method for real time quantitative RT-PCR.
journal, October 1996
- Gibson, U. E.; Heid, C. A.; Williams, P. M.
- Genome Research, Vol. 6, Issue 10
Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
journal, August 1991
- Holland, P. M.; Abramson, R. D.; Watson, R.
- Proceedings of the National Academy of Sciences, Vol. 88, Issue 16, p. 7276-7280
Molecular Beacons: Probes that Fluoresce upon Hybridization
journal, March 1996
- Tyagi, Sanjay; Kramer, Fred Russell
- Nature Biotechnology, Vol. 14, Issue 3, p. 303-308
DNA analysis and diagnostics on oligonucleotide microchips.
journal, May 1996
- Yershov, G.; Barsky, V.; Belgovskiy, A.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 10
Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer.
journal, December 1988
- Cardullo, R. A.; Agrawal, S.; Flores, C.
- Proceedings of the National Academy of Sciences, Vol. 85, Issue 23
Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.
journal, June 1995
- Livak, K. J.; Flood, S. J.; Marmaro, J.
- Genome Research, Vol. 4, Issue 6
Genetic Bit Analysis: a solid phase method for typing single nucleotide polymorphisms
journal, January 1994
- Nikiforov, Theo T.; Rendie, Robert B.; Goelet, Philip
- Nucleic Acids Research, Vol. 22, Issue 20
Automated DNA diagnostics using an ELISA-based oligonucleotide ligation assay.
journal, November 1990
- Nickerson, D. A.; Kaiser, R.; Lappin, S.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 22