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Title: Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer

Abstract

A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.

Inventors:
 [1];  [2]
  1. Clayton, MO
  2. St. Louis, MO
Issue Date:
Research Org.:
Univ. of Washington, Seattle, WA (United States)
OSTI Identifier:
872487
Patent Number(s):
5945283
Assignee:
Washington University (St. Louis, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG06-94ER61909
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
methods; kits; nucleic; acid; analysis; fluorescence; resonance; energy; transfer; method; detecting; presence; target; nucleotide; sequence; nucleotides; disclosed; comprised; forming; oligonucleotide; labeled; fluorophores; site; doubly; formed; addition; singly; dideoxynucleoside; triphosphate; polynucleotide; ligation; polynucleotides; detection; denaturation; indicates; provided; particularly; applicable; genotyping; resonance energy; energy transfer; particularly applicable; nucleic acid; target nucleotide; target site; acid target; nucleoside triphosphate; /435/436/

Citation Formats

Kwok, Pui-Yan, and Chen, Xiangning. Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer. United States: N. p., 1999. Web.
Kwok, Pui-Yan, & Chen, Xiangning. Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer. United States.
Kwok, Pui-Yan, and Chen, Xiangning. Fri . "Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer". United States. https://www.osti.gov/servlets/purl/872487.
@article{osti_872487,
title = {Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer},
author = {Kwok, Pui-Yan and Chen, Xiangning},
abstractNote = {A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1999},
month = {1}
}

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