Modulated excitation fluorescence analysis
Abstract
Fluorescence, spectroscopy is used to analyze small numbers of molecules that are present in a relatively small detection volume or zone. Information regarding physical and chemical properties of these molecules is determined by rapidly modulating the wavelength, intensity and/or polarization of laser energy to excite fluorophores that are attached either to the molecule of interest or a molecule that interacts with the molecule of interest. The emission profile of the fluorophores is used to determine useful information about the labeled and/or non-labeled molecules including molecular interactions between the molecules.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531572
- Patent Number(s):
- 7456954
- Application Number:
- 10/561,448
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG03-02ER63339
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2004-06-17
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; 42 ENGINEERING
Citation Formats
Weiss, Shimon, Kapanidis, Achillefs, Laurence, Ted A., and Lee, Nam K. Modulated excitation fluorescence analysis. United States: N. p., 2008.
Web.
Weiss, Shimon, Kapanidis, Achillefs, Laurence, Ted A., & Lee, Nam K. Modulated excitation fluorescence analysis. United States.
Weiss, Shimon, Kapanidis, Achillefs, Laurence, Ted A., and Lee, Nam K. Tue .
"Modulated excitation fluorescence analysis". United States. https://www.osti.gov/servlets/purl/1531572.
@article{osti_1531572,
title = {Modulated excitation fluorescence analysis},
author = {Weiss, Shimon and Kapanidis, Achillefs and Laurence, Ted A. and Lee, Nam K.},
abstractNote = {Fluorescence, spectroscopy is used to analyze small numbers of molecules that are present in a relatively small detection volume or zone. Information regarding physical and chemical properties of these molecules is determined by rapidly modulating the wavelength, intensity and/or polarization of laser energy to excite fluorophores that are attached either to the molecule of interest or a molecule that interacts with the molecule of interest. The emission profile of the fluorophores is used to determine useful information about the labeled and/or non-labeled molecules including molecular interactions between the molecules.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 25 00:00:00 EST 2008},
month = {Tue Nov 25 00:00:00 EST 2008}
}
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Works referenced in this record:
Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution
journal, April 1997
- Schwille, P.; Meyer-Almes, F. J.; Rigler, R.
- Biophysical Journal, Vol. 72, Issue 4, p. 1878-1886
Homogeneous Fluorescence Assay for Cyclic AMP
journal, June 2003
- Heyduk, Ewa; Fei, Ying; Heyduk, Tomasz
- Combinatorial Chemistry & High Throughput Screening, Vol. 6, Issue 4
Oligomerization of NTRC at the glnA enhancer is required for transcriptional activation.
journal, November 1993
- Porter, S. C.; North, A. K.; Wedel, A. B.
- Genes & Development, Vol. 7, Issue 11
Molecular beacons for detecting DNA binding proteins
journal, February 2002
- Heyduk, Tomasz; Heyduk, Ewa
- Nature Biotechnology, Vol. 20, Issue 2
On the analysis of high order moments of fluorescence fluctuations
journal, February 1990
- Qian, H.; Elson, E. L.
- Biophysical Journal, Vol. 57, Issue 2
A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
journal, February 2000
- Uetz, Peter; Giot, Loic; Cagney, Gerard
- Nature, Vol. 403, Issue 6770
System for distinguishing fluorescent molecule groups by time resolved fluorescence measurement
patent, October 2000
- Muller, Ralph; Sauer, Markus; Zander, Christoph
- US Patent Document 6,140,048
A comprehensive two-hybrid analysis to explore the yeast protein interactome
journal, March 2001
- Ito, T.; Chiba, T.; Ozawa, R.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 8
Correlating Structural Dynamics and Function in Single Ribozyme Molecules
journal, May 2002
- Zhuang, X.
- Science, Vol. 296, Issue 5572
ANALYTICAL CHEMISTRY: How to Detect Weak Pairs
journal, January 2003
- Laurence, T. A.
- Science, Vol. 299, Issue 5607
Fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin α-bungarotoxin with Torpedo californica acetylcholine receptor
journal, January 1996
- Rauer, Barbara; Neumann, Eberhard; Widengren, Jerker
- Biophysical Chemistry, Vol. 58, Issue 1-2
Simultaneous photometering method and assembly for multi-dimensional measurements concerning biologically related materials
patent, July 1979
- Wada, Akiyoshi
- US Patent Document 4,162,851
Towards a truly integrative biology through the functional genomics of yeast
journal, February 2001
- Delneri, Daniela; Brancia, Francesco L.; Oliver, Stephen G.
- Current Opinion in Biotechnology, Vol. 12, Issue 1
Simultaneous two-photon excitation of distinct labels for dual-color fluorescence crosscorrelation analysis
journal, September 2000
- Heinze, K. G.; Koltermann, A.; Schwille, P.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 19
The Transcription Initiation Pathway of Sigma 54 Mutants That Bypass the Enhancer Protein Requirement: IMPLICATIONS FOR THE MECHANISM OF ACTIVATION
journal, December 1996
- Wang, Jonathan T.; Gralla, Jay D.
- Journal of Biological Chemistry, Vol. 271, Issue 51
Quantitative Identification of Different Single Molecules by Selective Time-Resolved Confocal Fluorescence Spectroscopy
journal, August 1998
- Fries, Joachim R.; Brand, Leif; Eggeling, Christian
- The Journal of Physical Chemistry A, Vol. 102, Issue 33
Unusual Oligomerization Required for Activity of NtrC, a Bacterial Enhancer-Binding Protein
journal, March 1997
- Wyman, C.; Rombel, I.; North, A. K.
- Science, Vol. 275, Issue 5306
New Biarsenical Ligands and Tetracysteine Motifs for Protein Labeling in Vitro and in Vivo: Synthesis and Biological Applications
journal, May 2002
- Adams, Stephen R.; Campbell, Robert E.; Gross, Larry A.
- Journal of the American Chemical Society, Vol. 124, Issue 21
Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy
journal, February 1998
- Eggeling, C.; Fries, J. R.; Brand, L.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 4
Simultaneous Binding of Two DNA Duplexes to the NtrC−Enhancer Complex Studied by Two-Color Fluorescence Cross-Correlation Spectroscopy †
journal, March 2000
- Rippe, Karsten
- Biochemistry, Vol. 39, Issue 9
Ultrahigh-resolution multicolor colocalization of single fluorescent probes
journal, August 2000
- Lacoste, T. D.; Michalet, X.; Pinaud, F.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 17, p. 9461-9466
Fluorescence-aided molecule sorting: Analysis of structure and interactions by alternating-laser excitation of single molecules
journal, June 2004
- Kapanidis, Achillefs N.; Lee, Nam Ki; Laurence, Ted A.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 24
MgATP Binding and Hydrolysis Determinants of NtrC, a Bacterial Enhancer-Binding Protein
journal, August 1999
- Rombel, Irene; Peters-Wendisch, Petra; Mesecar, Andrew
- Journal of Bacteriology, Vol. 181, Issue 15
Method for differentiating or detecting particles in a sample by identifying signal segments of time-resolved, optical raw signals from the sample on the basis of single photon detection
patent, March 2001
- Seidel, Claus; Günther, Rolf W.; Lupke, Stefan A.
- US Patent Document 6,208,815
Photobleaching of Fluorescent Dyes under Conditions Used for Single-Molecule Detection: Evidence of Two-Step Photolysis
journal, July 1998
- Eggeling, C.; Widengren, J.; Rigler, R.
- Analytical Chemistry, Vol. 70, Issue 13
Effect of A(n) tracts within the UP element proximal subsite of a model promoter on kinetics of open complex formation by Escherichia coli RNA polymerase.
journal, September 2002
- Kolasa, Iwona K.; Loziński, Tomasz; Wierzchowski, Kazimierz L.
- Acta Biochimica Polonica, Vol. 49, Issue 3
Specific Labeling of Polypeptides at Amino-Terminal Cysteine Residues Using Cy5-benzyl Thioester
journal, September 2002
- Schuler, Benjamin; Pannell, Lewis K.
- Bioconjugate Chemistry, Vol. 13, Issue 5
Determination of the diffusion coefficient of dye in solution at single molecule level
journal, April 1997
- Ko, D. -S.; Sauer, M.; Nord, S.
- Chemical Physics Letters, Vol. 269, Issue 1-2
Proteome, transcriptome and genome: top down or bottom up analysis?
journal, November 2001
- Kettman, John R.; Frey, Johann R.; Lefkovits, Ivan
- Biomolecular Engineering, Vol. 18, Issue 5
New energy transfer dyes for DNA sequencing
journal, July 1997
- Lee, L. G.; Spurgeon, S. L.; Heiner, C.R.
- Nucleic Acids Research, Vol. 25, Issue 14, p. 2816-2822
Single-channel multicolored correlation analysis
patent, May 2007
- Rigler, Rudolf; Thyberg, Per; Honegger, Adrian
- US Patent Document 7,223,985
Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase
journal, October 2002
- Ha, Taekjip; Rasnik, Ivan; Cheng, Wei
- Nature, Vol. 419, Issue 6907
Method for detecting reactions by means of coincidence analysis
patent, March 2001
- Eigen, Manfred; Winkler, Thorsten; Stephan, Jens
- US Patent Document 6,200,818
Ligand-induced conformational changes observed in single RNA molecules
journal, August 1999
- Ha, T.; Zhuang, X.; Kim, H. D.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 16
PROTEIN BIOCHEMISTRY:Protein Interaction Methods-Toward an Endgame
journal, June 1999
- Mendelsohn, A. R.
- Science, Vol. 284, Issue 5422
FRET Study of a Trifluorophore-Labeled DNAzyme
journal, December 2002
- Liu, Juewen; Lu, Yi
- Journal of the American Chemical Society, Vol. 124, Issue 51
Ultrasensitive hybridization analysis using fluorescence correlation spectroscopy
journal, May 1995
- Kinjo, M.; Rigler, R.
- Nucleic Acids Research, Vol. 23, Issue 10
Methods of screening for ligands of target molecules
patent-application, March 2003
- Djaballah, Hakim; Rongey, Scott; Patel, Rupal
- US Patent Application 10/170758; 20030059811
Luminescence assay using cyclical excitation wavelength sequence
patent, September 2002
- Morgan, Christopher G.
- US Patent Document 6,444,476
Ratiometric measurement and identification of single diffusing molecules
journal, August 1999
- Dahan, Maxime; Deniz, Ashok A.; Ha, Taekjip
- Chemical Physics, Vol. 247, Issue 1
Quantitative Hybridization Kinetics of DNA Probes to RNA in Solution Followed by Diffusional Fluorescence Correlation Analysis †
journal, January 1996
- Schwille, Petra; Oehlenschläger, Frank; Walter, Nils G.
- Biochemistry, Vol. 35, Issue 31
Two-Dimensional Fluorescence Intensity Distribution Analysis: Theory and Applications
journal, April 2000
- Kask, Peet; Palo, Kaupo; Fay, Nicolas
- Biophysical Journal, Vol. 78, Issue 4
Scanning force microscopy of Escherichia coli RNA polymerase·σ 54 holoenzyme complexes with DNA in buffer and in air 1 1Edited by W. Baumeister
journal, November 1998
- Schulz, Alexandra; Mücke, Norbert; Langowski, Jörg
- Journal of Molecular Biology, Vol. 283, Issue 4
Role of integration host factor in stimulating transcription from the σ54-dependent nifH promoter
journal, October 1992
- Santero, Eduardo; Hoover, Timothy R.; North, Anne K.
- Journal of Molecular Biology, Vol. 227, Issue 3
Confocal microscope for optical determination of an observation volume
patent, September 2000
- Kask, Peet; Hummel, Stefan
- US Patent Document 6,122,098
Transcriptional activation via DNA-looping: visualization of intermediates in the activation pathway of E. coli RNA polymerase·σ54 holoenzyme by scanning force microscopy
journal, July 1997
- Rippe, Karsten; Guthold, Martin; von Hippel, Peter H.
- Journal of Molecular Biology, Vol. 270, Issue 2
Method of characterizing fluorescent molecules or other particles using generating functions
patent, April 2002
- Palo, Kaupo
- US Patent Document 6,376,843
Method for characterizing samples by determination of a function of at least one specific property of particles in a sample
patent, April 2003
- Palo, Kaupo
- US Patent Document 6,556,296
Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library
journal, January 2003
- Feldhaus, Michael J.; Siegel, Robert W.; Opresko, Lee K.
- Nature Biotechnology, Vol. 21, Issue 2
Method and device for determining predetermined properties of target particles of a sample medium
patent, October 2000
- Seidel, Claus; Brand, Leif; Günther, Rolf W.
- US Patent Document 6,137,584
Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.
journal, June 1996
- Ha, T.; Enderle, T.; Ogletree, D. F.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 13
Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy
journal, October 2002
- Schuler, Benjamin; Lipman, Everett A.; Eaton, William A.
- Nature, Vol. 419, Issue 6908
Fluorescence-intensity distribution analysis and its application in biomolecular detection technology
journal, November 1999
- Kask, P.; Palo, K.; Ullmann, D.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 24
Guilt-by-association goes global
journal, February 2000
- Oliver, Stephen
- Nature, Vol. 403, Issue 6770
Zero-Mode Waveguides for Single-Molecule Analysis at High Concentrations
journal, January 2003
- Levene, M. J.
- Science, Vol. 299, Issue 5607
High speed digital imaging microscope
patent, August 1993
- Bowman, Douglas R.; Fay, Fredric S.; Rodgers, Cyril
- US Patent Document 5,233,197
MINOR GROOVE-BINDING ARCHITECTURAL PROTEINS: Structure, Function, and DNA Recognition
journal, June 1998
- Bewley, Carole A.; Gronenborn, Angela M.; Clore, G. Marius
- Annual Review of Biophysics and Biomolecular Structure, Vol. 27, Issue 1
Multiparameter single-molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptase:primer/template complexes
journal, February 2003
- Rothwell, P. J.; Berger, S.; Kensch, O.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 4
Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism
journal, February 1999
- Ha, T.; Ting, A. Y.; Liang, J.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 3
Single-pair fluorescence resonance energy transfer on freely diffusing molecules: Observation of Forster distance dependence and subpopulations
journal, March 1999
- Deniz, A. A.; Dahan, M.; Grunwell, J. R.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 7
Association states of the transcription activator protein NtrC from E. coli determined by analytical ultracentrifugation 1 1Edited by K. Yamamoto
journal, May 1998
- Rippe, Karsten; Mücke, Norbert; Schulz, Alexandra
- Journal of Molecular Biology, Vol. 278, Issue 5
Gene and Protein Expression Profiles of Shewanella oneidensis during Anaerobic Growth with Different Electron Acceptors
journal, January 2002
- Beliaev, Alex S.; Thompson, Dorothea K.; Khare, Tripti
- OMICS: A Journal of Integrative Biology, Vol. 6, Issue 1
Phosphorylation-Induced Signal Propagation in the Response Regulator NtrC
journal, September 2000
- Lee, Jonghui; Owens, Jeffrey T.; Hwang, Ingyu
- Journal of Bacteriology, Vol. 182, Issue 18
DNA-looping and enhancer activity: association between DNA-bound NtrC activator and RNA polymerase at the bacterial glnA promoter.
journal, July 1990
- Su, W.; Porter, S.; Kustu, S.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 14
Networking proteins in yeast
journal, April 2001
- Hazbun, T. R.; Fields, S.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 8
Fluorescence Intensity Multiple Distributions Analysis: Concurrent Determination of Diffusion Times and Molecular Brightness
journal, December 2000
- Palo, Kaupo; Mets, Ülo; Jäger, Stefan
- Biophysical Journal, Vol. 79, Issue 6
Method for characterizing samples on the basis of intermediate statistical data
patent, February 2003
- Kask, Peet
- US Patent Document 6,515,289
Abortive Cycling and the Release of Polymerase for Elongation at the σ54-dependent glnAp2 Promoter
journal, October 1995
- Tintut, Yin; Wang, Jonathan T.; Gralla, Jay D.
- Journal of Biological Chemistry, Vol. 270, Issue 41
Fluorescent probes and bioconjugation chemistries for single-molecule fluorescence analysis of biomolecules
journal, December 2002
- Kapanidis, Achillefs N.; Weiss, Shimon
- The Journal of Chemical Physics, Vol. 117, Issue 24
Confocal microscope for optical determination of an observation volume
patent, June 2002
- Kask, Peet; Hummel, Stefan
- US Patent Document 6,407,856
Molecular beacons for detecting DNA binding proteins: mechanism of action
journal, May 2003
- Heyduk, Ewa; Knoll, Eric; Heyduk, Tomasz
- Analytical Biochemistry, Vol. 316, Issue 1
Site-Specific Incorporation of Fluorescent Probes into Protein: Hexahistidine-Tag-Mediated Fluorescent Labeling with (Ni 2+ :Nitrilotriacetic Acid) n −Fluorochrome Conjugates
journal, December 2001
- Kapanidis, Achillefs N.; Ebright, Yon W.; Ebright, Richard H.
- Journal of the American Chemical Society, Vol. 123, Issue 48
Method for measuring the association of substructures of pathological protein depositions
patent, December 2002
- Riesner, Detlev; Post, Kai; Schaefer, Oliver
- US Patent Document 6,498,017
Thermodynamic Fluctuations in a Reacting System—Measurement by Fluorescence Correlation Spectroscopy
journal, September 1972
- Magde, Douglas; Elson, Elliot; Webb, W. W.
- Physical Review Letters, Vol. 29, Issue 11
Semiconductor Nanocrystals as Fluorescent Biological Labels
patent, September 1998
- Bruchez Jr., Marcel; Moronne, Mario Moronne; Gin, Peter Gin
- Science, Vol. 281, Issue 5385, p. 2013-2016
Genome-wide protein interaction maps using two-hybrid systems
journal, August 2000
- Legrain, Pierre; Selig, Luc
- FEBS Letters, Vol. 480, Issue 1
Two excitation wavelength video imaging microscope
patent, September 1992
- Fay, Frederic; Dellaville, Fernando
- US Patent Document 5,149,972
Analysis of the Shewanella oneidensis proteome by two-dimensional gel electrophoresis under nondenaturing conditions
journal, May 2003
- Giometti, Carol S.; Khare, Tripti; Tollaksen, Sandra L.
- PROTEOMICS, Vol. 3, Issue 5
Method and apparatus for identifying fluorophores
patent, July 1998
- Gorfinkel, Vera; Luryi, Serge
- US Patent Document 5,784,157
NEW EMBO MEMBER'S REVIEW: Diversity of protein-protein interactions
journal, July 2003
- Nooren, I. M. A.
- The EMBO Journal, Vol. 22, Issue 14
Global structure similarities of intact and nicked DNA complexed with IHF measured in solution by fluorescence resonance energy transfer
journal, December 1999
- Lorenz, M.
- Nucleic Acids Research, Vol. 27, Issue 23
A protease assay for two-photon crosscorrelation and FRET analysis based solely on fluorescent proteins
journal, September 2002
- Kohl, T.; Heinze, K. G.; Kuhlemann, R.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 19
Method and device for the determination of material-specific parameters of one or a few molecules by means of correlation spectroscopy
patent, August 1999
- Günther, Rolf W.
- US Patent Document 5,933,233
Method for direct identification of few nucleic acid strands
patent, September 1998
- Eigen, Manfred; Rigler, Rudolf
- US Patent Document 5,807,677
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- Mehanian, Courosh; Hansen, Hans J.; Wang, Yingjian
- US Patent Document 7,782,452
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