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Title: Shaped-excitation fluorescence (SEF) microscopy

Abstract

A method and apparatus for detecting more than one fluorophore in a sample. A broad spectrum excitation light is passed through a shaping filter before illuminating the target sample with more than one fluorophore to be detected, where the optical density of the shaping filter changes based on the wavelength of the broad-spectrum excitation light passing through it.

Inventors:
Issue Date:
Research Org.:
Bowling Green State Univ., Bowling Green, OH (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1771639
Patent Number(s):
10837910
Application Number:
16/245,241
Assignee:
Bowling Green State University (Bowling Green, OH)
Patent Classifications (CPCs):
G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
SC0016872
Resource Type:
Patent
Resource Relation:
Patent File Date: 01/10/2019
Country of Publication:
United States
Language:
English

Citation Formats

Zamkov, Mikhail. Shaped-excitation fluorescence (SEF) microscopy. United States: N. p., 2020. Web.
Zamkov, Mikhail. Shaped-excitation fluorescence (SEF) microscopy. United States.
Zamkov, Mikhail. Tue . "Shaped-excitation fluorescence (SEF) microscopy". United States. https://www.osti.gov/servlets/purl/1771639.
@article{osti_1771639,
title = {Shaped-excitation fluorescence (SEF) microscopy},
author = {Zamkov, Mikhail},
abstractNote = {A method and apparatus for detecting more than one fluorophore in a sample. A broad spectrum excitation light is passed through a shaping filter before illuminating the target sample with more than one fluorophore to be detected, where the optical density of the shaping filter changes based on the wavelength of the broad-spectrum excitation light passing through it.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {11}
}

Works referenced in this record:

Fluorescent Indicators for Intracellular pH
journal, May 2010


Improving the spectral resolution in fluorescence microscopy through shaped-excitation imaging
journal, August 2018


Advances in fluorescence labeling strategies for dynamic cellular imaging
journal, June 2014