System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

Demos, Stavros; Levenson, Richard

Title: System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

Abstract

The present disclosure relates to a method for analyzing tissue specimens. In one implementation the method involves obtaining a tissue sample and exposing the sample to one or more fluorophores as contrast agents to enhance contrast of subcellular compartments of the tissue sample. The tissue sample is illuminated by an ultraviolet (UV) light having a wavelength between about 200 nm to about 400 nm, with the wavelength being selected to result in penetration to only a specified depth below a surface of the tissue sample. Inter-image operations between images acquired under different imaging parameters allow for improvement of the image quality via removal of unwanted image components. A microscope may be used to image the tissue sample and provide the image to an image acquisition system that makes use of a camera. The image acquisition system may create a corresponding image that is transmitted to a display system for processing and display.

Inventors:: Demos, Stavros; Levenson, Richard

Issue Date:: 2017-04-18

Research Org.:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1351831

Patent Number(s):: 9625387

Application Number:: 14/487,997

Assignee:: Lawrence Livermore National Security, LLC

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2201/062 - LED's
G01N21/6458 - {Fluorescence microscopy

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS
A61B5/0071 - {by measuring fluorescence emission}

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2021/6421 - {Measuring at two or more wavelengths}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS
A61B5/0044 - {for the heart}

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N21/6445 - {Measuring fluorescence polarisation}
G01N2021/6441 - {with two or more labels}
G01N21/6428 - {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
G01N2021/6419 - {Excitation at two or more wavelengths}
G01N21/6486 - {Measuring fluorescence of biological material, e.g. DNA, RNA, cells

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DOE Contract Number:: AC52-07NA27344

Resource Type:: Patent

Resource Relation:: Patent File Date: 2014 Sep 16

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 36 MATERIALS SCIENCE

Citation Formats


                    Demos, Stavros, and Levenson, Richard. System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents.  United States: N. p., 2017. 
        Web.

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                    Demos, Stavros, & Levenson, Richard. System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents.  United States.

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                    Demos, Stavros, and Levenson, Richard. Tue .  
        "System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents".  United States.  https://www.osti.gov/servlets/purl/1351831.

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@article{osti_1351831,

  title        = {System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents},

  author       = {Demos, Stavros and Levenson, Richard},

  abstractNote = {The present disclosure relates to a method for analyzing tissue specimens. In one implementation the method involves obtaining a tissue sample and exposing the sample to one or more fluorophores as contrast agents to enhance contrast of subcellular compartments of the tissue sample. The tissue sample is illuminated by an ultraviolet (UV) light having a wavelength between about 200 nm to about 400 nm, with the wavelength being selected to result in penetration to only a specified depth below a surface of the tissue sample. Inter-image operations between images acquired under different imaging parameters allow for improvement of the image quality via removal of unwanted image components. A microscope may be used to image the tissue sample and provide the image to an image acquisition system that makes use of a camera. The image acquisition system may create a corresponding image that is transmitted to a display system for processing and display.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2017},

  month        = {4}

}

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Works referenced in this record:

Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation
journal, January 2009

Lin, Bevin; Urayama, Shiro; Saroufeem, Ramez M. G.
Optics Express, Vol. 17, Issue 15, p. 12502-12509
https://doi.org/10.1364/OE.17.012502

Label-Free Bacterial Imaging with Deep-UV-Laser-Induced Native Fluorescence
journal, September 2010

Bhartia, R.; Salas, E. C.; Hug, W. F.
Applied and Environmental Microbiology, Vol. 76, Issue 21, p. 7231-7237
https://doi.org/10.1128/AEM.00943-10

Deep UV autofluorescence microscopy for cell biology and tissue histology Deep UV autofluorescence microscopy
journal, April 2013

Jamme, Frédéric; Kascakova, Slavka; Villette, Sandrine
Biology of the Cell, Vol. 105, Issue 7, p. 277-288
https://doi.org/10.1111/boc.201200075

Deep-UV fluorescence lifetime imaging microscopy
journal, January 2015

de Jong, Christiaan J.; Lajevardipour, Alireza; Gecevičius, Mindaugas
Photonics Research, Vol. 3, Issue 5, p. 283-288
https://doi.org/10.1364/PRJ.3.000283

Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imaging
conference, March 2015

Fereidouni, Farzad; Datta-Mitra, Ananya; Demos, Stavros
https://doi.org/10.1117/12.2080408

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Title: System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

Abstract

Citation Formats

Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation journal, January 2009

Label-Free Bacterial Imaging with Deep-UV-Laser-Induced Native Fluorescence journal, September 2010

Deep UV autofluorescence microscopy for cell biology and tissue histology Deep UV autofluorescence microscopy journal, April 2013

Deep-UV fluorescence lifetime imaging microscopy journal, January 2015

Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imaging conference, March 2015

Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation
journal, January 2009

Label-Free Bacterial Imaging with Deep-UV-Laser-Induced Native Fluorescence
journal, September 2010

Deep UV autofluorescence microscopy for cell biology and tissue histology Deep UV autofluorescence microscopy
journal, April 2013

Deep-UV fluorescence lifetime imaging microscopy
journal, January 2015

Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imaging
conference, March 2015