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Title: System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

The present disclosure relates to a method for analyzing tissue specimens. In one implementation the method involves obtaining a tissue sample and exposing the sample to one or more fluorophores as contrast agents to enhance contrast of subcellular compartments of the tissue sample. The tissue sample is illuminated by an ultraviolet (UV) light having a wavelength between about 200 nm to about 400 nm, with the wavelength being selected to result in penetration to only a specified depth below a surface of the tissue sample. Inter-image operations between images acquired under different imaging parameters allow for improvement of the image quality via removal of unwanted image components. A microscope may be used to image the tissue sample and provide the image to an image acquisition system that makes use of a camera. The image acquisition system may create a corresponding image that is transmitted to a display system for processing and display.
Issue Date:
OSTI Identifier:
Lawrence Livermore National Security, LLC LLNL
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2014 Sep 16
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
Country of Publication:
United States

Other works cited in this record:

Systems and methods for the multispectral imaging and characterization of skin tissue
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Real-time microscopic imaging of esophageal epithelial disease with autofluorescence under ultraviolet excitation
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journal, January 2015
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Microscopy with UV Surface Excitation (MUSE) for slide-free histology and pathology imaging
conference, March 2015

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