skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping

Abstract

A microscopy imaging system is disclosed that includes a light source system, a spectral shaper, a modulator system, an optics system, an optical detector and a processor. The light source system is for providing a first train of pulses and a second train of pulses. The spectral shaper is for spectrally modifying an optical property of at least some frequency components of the broadband range of frequency components such that the broadband range of frequency components is shaped producing a shaped first train of pulses to specifically probe a spectral feature of interest from a sample, and to reduce information from features that are not of interest from the sample. The modulator system is for modulating a property of at least one of the shaped first train of pulses and the second train of pulses at a modulation frequency. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of a train of pulses of interest transmitted or reflected through the common focal volume. The processor is for detecting a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the train of pulses of interest duemore » to the non-linear interaction of the shaped first train of pulses with the second train of pulses as modulated in the common focal volume, and for providing an output signal for a pixel of an image for the microscopy imaging system.« less

Inventors:
; ;
Issue Date:
Research Org.:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1243043
Patent Number(s):
9285575
Application Number:
12/690,579
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
DOE Contract Number:  
FG02-07ER15875
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Jan 20
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Xie, Xiaoliang Sunney, Freudiger, Christian, and Min, Wei. Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping. United States: N. p., 2016. Web.
Xie, Xiaoliang Sunney, Freudiger, Christian, & Min, Wei. Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping. United States.
Xie, Xiaoliang Sunney, Freudiger, Christian, and Min, Wei. Tue . "Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping". United States. https://www.osti.gov/servlets/purl/1243043.
@article{osti_1243043,
title = {Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping},
author = {Xie, Xiaoliang Sunney and Freudiger, Christian and Min, Wei},
abstractNote = {A microscopy imaging system is disclosed that includes a light source system, a spectral shaper, a modulator system, an optics system, an optical detector and a processor. The light source system is for providing a first train of pulses and a second train of pulses. The spectral shaper is for spectrally modifying an optical property of at least some frequency components of the broadband range of frequency components such that the broadband range of frequency components is shaped producing a shaped first train of pulses to specifically probe a spectral feature of interest from a sample, and to reduce information from features that are not of interest from the sample. The modulator system is for modulating a property of at least one of the shaped first train of pulses and the second train of pulses at a modulation frequency. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of a train of pulses of interest transmitted or reflected through the common focal volume. The processor is for detecting a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the train of pulses of interest due to the non-linear interaction of the shaped first train of pulses with the second train of pulses as modulated in the common focal volume, and for providing an output signal for a pixel of an image for the microscopy imaging system.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {3}
}

Patent:

Save / Share:

Works referenced in this record:

Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
journal, December 2008


Low-Cost Spectroscopy with a Variable Multivariate Optical Element
journal, October 2006


Enhancing two-color absorption, self-phase modulation, and Raman microscopy signatures in tissue with femtosecond laser pulse shaping
conference, March 2009


Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy
journal, January 2009


Generation of narrow-bandwidth picosecond visible pulses from broadband femtosecond pulses for femtosecond stimulated Raman
journal, September 2006


Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy and Study of Lipid Vesicles
journal, August 2002


Single pulse interferometric coherent anti-Stokes Raman scattering (CARS)
conference, February 2007


Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
journal, May 1999


Femtosecond stimulated Raman microscopy
journal, April 2007


Generalization of multivariate optical computations as a method for improving the speed and precision of spectroscopic analyses
journal, January 2008