Rapid microfluidic thermal cycler for nucleic acid amplification
Abstract
A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1224444
- Patent Number(s):
- 9170060
- Application Number:
- 12/270,348
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
F - MECHANICAL ENGINEERING F28 - HEAT EXCHANGE IN GENERAL F28F - DETAILS OF HEAT-EXCHANGE AND HEAT-TRANSFER APPARATUS, OF GENERAL APPLICATION
- DOE Contract Number:
- AC52-07NA27344
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2008 Nov 13
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 42 ENGINEERING
Citation Formats
Beer, Neil Reginald, and Vafai, Kambiz. Rapid microfluidic thermal cycler for nucleic acid amplification. United States: N. p., 2015.
Web.
Beer, Neil Reginald, & Vafai, Kambiz. Rapid microfluidic thermal cycler for nucleic acid amplification. United States.
Beer, Neil Reginald, and Vafai, Kambiz. Tue .
"Rapid microfluidic thermal cycler for nucleic acid amplification". United States. https://www.osti.gov/servlets/purl/1224444.
@article{osti_1224444,
title = {Rapid microfluidic thermal cycler for nucleic acid amplification},
author = {Beer, Neil Reginald and Vafai, Kambiz},
abstractNote = {A system for thermal cycling a material to be thermal cycled including a microfluidic heat exchanger; a porous medium in the microfluidic heat exchanger; a microfluidic thermal cycling chamber containing the material to be thermal cycled, the microfluidic thermal cycling chamber operatively connected to the microfluidic heat exchanger; a working fluid at first temperature; a first system for transmitting the working fluid at first temperature to the microfluidic heat exchanger; a working fluid at a second temperature, a second system for transmitting the working fluid at second temperature to the microfluidic heat exchanger; a pump for flowing the working fluid at the first temperature from the first system to the microfluidic heat exchanger and through the porous medium; and flowing the working fluid at the second temperature from the second system to the heat exchanger and through the porous medium.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 27 00:00:00 EDT 2015},
month = {Tue Oct 27 00:00:00 EDT 2015}
}
Works referenced in this record:
Process for amplifying, detecting, and/or-cloning nucleic acid sequences
patent, July 1987
- Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman
- US Patent Document 4,683,195
Process for amplifying nucleic acid sequences
patent, July 1987
- Mullis, Kary B.
- US Patent Document 4,683,202
End-complementary polymerase reaction
patent, November 1998
- Stemmer, Willem P.; Lipshutz, Robert J.
- US Patent Document 5,834,252
Method for recombining polynucleotides
patent-application, August 2002
- Slater, Steven C.; Ng, Wing-On; Feng, Guo Hong
- US Patent Application 09/746432; 20020119535
Method for the complete chemical synthesis and assembly of genes and genomes
patent-application, September 2003
- Evans, Glen A.
- US Patent Application 10/322360; 20030165946
Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
patent-application, June 2004
- Atwood, John Girdner; Mossa, Albert Carmelo
- US Patent Application 10/691186; 20040086927
Thermal reaction device and method for using the same
patent-application, November 2005
- McBride, Lincoln; Facer, Geoffrey; Unger, Marc
- US Patent Application 11/084357; 20050252773
Thermal reaction device and method for using the same
patent-application, November 2005
- McBride, Lincoln; Facer, Geoffrey; Unger, Marc
- US Patent Application 11/084357; 20050252773
Method for carrying out a biochemical protocol in continuous flow in a microreactor
patent-application, December 2005
- Fouillet, Yves; Vauchier, Claude; Clerc, Jean-Frederic
- US Patent Application 11/197040; 20050282224
Pumped fluid cooling system and method
patent-application, March 2006
- Werner, Douglas; Goodson, Kenneth; Munch, Mark
- US Patent Application 10/927800; 20060042785
Thermal cycler for microfluidic array assays
patent-application, May 2006
- Yoder, Karl; Brenan, Colin; Linton, John
- US Patent Application 11/227425; 20060094108
Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
patent-application, July 2008
- Beer, Neil; Hindson, Benjamin; Colson, Billy
- US Patent Application 11/650363; 20080166793
Portable Rapid Microfluidic Thermal Cycler for Extremely Fast Nucleic Acid Amplification
patent-application, September 2009
- Beer, Neil Reginald; Vafai, Kambiz; Mahjoob, Shadi
- US Patent Application 12/270030; 20090226971
Isothermal surface production and regulation for high heat flux applications utilizing porous inserts
journal, August 2001
- Khanafer, K.; Vafai, K.
- International Journal of Heat and Mass Transfer, Vol. 44, Issue 15, p. 2933-2947
Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification
journal, May 2008
- Mahjoob, Shadi; Vafai, Kambiz; Beer, N. Reginald
- International Journal of Heat and Mass Transfer, Vol. 51, Issue 9-10, p. 2109-2122
On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets
journal, March 2008
- Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna
- Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858