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Title: Multi-chamber nucleic acid amplification and detection device

A nucleic acid amplification and detection device includes an amplification cartridge with a plurality of reaction chambers for containing an amplification reagent and a visual detection reagent, and a plurality of optically transparent view ports for viewing inside the reaction chambers. The cartridge also includes a sample receiving port which is adapted to receive a fluid sample and fluidically connected to distribute the fluid sample to the reaction chamber, and in one embodiment, a plunger is carried by the cartridge for occluding fluidic communication to the reaction chambers. The device also includes a heating apparatus having a heating element which is activated by controller to generate heat when a trigger event is detected. The heating apparatus includes a cartridge-mounting section which positioned a cartridge in thermal communication with the heating element so that visual changes to the contents of the reaction chambers are viewable through the view ports.
Inventors:
Issue Date:
OSTI Identifier:
1404944
Assignee:
Lawrence Livermore National Security, LLCq LLNL
Patent Number(s):
9,795,968
Application Number:
14/257,313
Contract Number:
AC52-07NA27344
Resource Relation:
Patent File Date: 2014 Apr 21
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 47 OTHER INSTRUMENTATION

Other works cited in this record:

Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
patent, December 1996

Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay
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Assay system and method for conducting assays
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Methods for conducting tests
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Flexible heating cover assembly for thermal cycling of samples of biological material
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Chemiluminescence-based microfluidic biochip
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Microstructured device for removable storage of small amounts of liquid and a process for removal of the liquid stored in this device
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Biosensor Chip, and Biosensor Device Equipped with Same
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Normally Closed Microwave and Applications of the Same
patent-application, October 2015

A simple and sensitive method for detection of Bacillus anthracis by loop-mediated isothermal amplification
journal, December 2009

Development and Initial Results of a Low Cost, Disposable, Point-of-Care Testing Device for Pathogen Detection
journal, October 2010
  • Bearinger, Jane P.; Dugan, Lawrence C.; Baker, Brian R.
  • IEEE Transactions on Biomedical Engineering, Vol. 58, Issue 3, p. 805-808
  • DOI: 10.1109/TBME.2010.2089054

LAVA: An Open-Source Approach To Designing LAMP (Loop-Mediated Isothermal Amplification) DNA Signatures
journal, June 2011
  • Torres, Clinton; Vitalis, Elizabeth A; Baker, Brian R
  • BMC Bioinformatics, Vol. 12, Issue 1, Article No: 240
  • DOI: 10.1186/1471-2105-12-240

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