skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit

Abstract

Multiplexed affinity purification and thermal dissociation prior to biochip hybridization simplifies uncharacterized sample admixtures, thereby minimizing or eliminating sample interferents, improving hybridization specificity on a microarray detector, and minimizing or eliminating the need for post-hybridization thermal dissociation analysis. An integrated thermo-affinity sample preparation sub-circuit for sample purification and enrichment is described that is consistent with a field-portable form factor and analytical processes. Thermo-affinity sample preparation on model admixtures of varying complexity was efficacious.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531688
Patent Number(s):
7,985,716
Application Number:
11/744,112
Assignee:
UChicago Argonne, LLC (Chicago, IL)
DOE Contract Number:  
AC02-06CH11357; W-31-109-ENG-38
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007-05-03
Country of Publication:
United States
Language:
English

Citation Formats

Yershov, Gennadiy M., Kukhtin, Alexander, Chernov, Boris K., Golova, Julia B., and Chandler, Darrell P. Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit. United States: N. p., 2011. Web.
Yershov, Gennadiy M., Kukhtin, Alexander, Chernov, Boris K., Golova, Julia B., & Chandler, Darrell P. Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit. United States.
Yershov, Gennadiy M., Kukhtin, Alexander, Chernov, Boris K., Golova, Julia B., and Chandler, Darrell P. Tue . "Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit". United States. https://www.osti.gov/servlets/purl/1531688.
@article{osti_1531688,
title = {Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit},
author = {Yershov, Gennadiy M. and Kukhtin, Alexander and Chernov, Boris K. and Golova, Julia B. and Chandler, Darrell P.},
abstractNote = {Multiplexed affinity purification and thermal dissociation prior to biochip hybridization simplifies uncharacterized sample admixtures, thereby minimizing or eliminating sample interferents, improving hybridization specificity on a microarray detector, and minimizing or eliminating the need for post-hybridization thermal dissociation analysis. An integrated thermo-affinity sample preparation sub-circuit for sample purification and enrichment is described that is consistent with a field-portable form factor and analytical processes. Thermo-affinity sample preparation on model admixtures of varying complexity was efficacious.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {7}
}

Patent:

Save / Share:

Works referencing / citing this record:

Devices and methods for molecular diagnostic testing
patent, April 2017


Screening for structural variants
patent, September 2018


Variant database
patent, March 2016


Cartridge-based thermocycler
patent, February 2019


Devices and methods for molecular diagnostic testing
patent, November 2018


Devices and methods for molecular diagnostic testing
patent, August 2018


Molecular diagnostic device
patent, October 2017


Devices and methods for molecular diagnostic testing
patent, October 2018


Methods for analyzing nucleic acid
patent, February 2019


Devices and methods for molecular diagnostic testing
patent, October 2018