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Title: Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit

Abstract

Multiplexed affinity purification and thermal dissociation prior to biochip hybridization simplifies uncharacterized sample admixtures, thereby minimizing or eliminating sample interferents, improving hybridization specificity on a microarray detector, and minimizing or eliminating the need for post-hybridization thermal dissociation analysis. An integrated thermo-affinity sample preparation sub-circuit for sample purification and enrichment is described that is consistent with a field-portable form factor and analytical processes. Thermo-affinity sample preparation on model admixtures of varying complexity was efficacious.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1531688
Patent Number(s):
7985716
Application Number:
11/744,112
Assignee:
UChicago Argonne, LLC (Chicago, IL)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC02-06CH11357; W-31-109-ENG-38
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007-05-03
Country of Publication:
United States
Language:
English

Citation Formats

Yershov, Gennadiy M., Kukhtin, Alexander, Chernov, Boris K., Golova, Julia B., and Chandler, Darrell P. Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit. United States: N. p., 2011. Web.
Yershov, Gennadiy M., Kukhtin, Alexander, Chernov, Boris K., Golova, Julia B., & Chandler, Darrell P. Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit. United States.
Yershov, Gennadiy M., Kukhtin, Alexander, Chernov, Boris K., Golova, Julia B., and Chandler, Darrell P. Tue . "Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit". United States. https://www.osti.gov/servlets/purl/1531688.
@article{osti_1531688,
title = {Nucleic acid sample purification and enrichment with a thermo-affinity microfluidic sub-circuit},
author = {Yershov, Gennadiy M. and Kukhtin, Alexander and Chernov, Boris K. and Golova, Julia B. and Chandler, Darrell P.},
abstractNote = {Multiplexed affinity purification and thermal dissociation prior to biochip hybridization simplifies uncharacterized sample admixtures, thereby minimizing or eliminating sample interferents, improving hybridization specificity on a microarray detector, and minimizing or eliminating the need for post-hybridization thermal dissociation analysis. An integrated thermo-affinity sample preparation sub-circuit for sample purification and enrichment is described that is consistent with a field-portable form factor and analytical processes. Thermo-affinity sample preparation on model admixtures of varying complexity was efficacious.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {7}
}

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