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Title: Multiplexed microfluidic approach for nucleic acid enrichment

A system for enhancing a nucleic acid sample may include a one pump, a denaturing chamber; a microfluidic hydroxyapatite chromatography device configured for performing hydroxyapatite chromatography on the nucleic acid sample, a sample collector, and tubing connecting the pump with the denaturing chamber, the hydroxyapatite chromatography device and the sample collector such that the pump may be used to move the nucleic acid sample from the denaturing chamber to the hydroxyapatite chromatography device and then to the sample collector.
Inventors:
; ; ; ; ; ; ; ;
Issue Date:
OSTI Identifier:
1248895
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
Patent Number(s):
9,322,014
Application Number:
14/084,937
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2013 Nov 20
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Works referenced in this record:

Rare target enrichment for ultrasensitive PCR detection using cot–rehybridization and duplex-specific nuclease
journal, February 2012
  • Gijavanekar, Charul; Strych, Ulrich; Fofanov, Yuriy
  • Analytical Biochemistry, Vol. 421, Issue 1, p. 81-85
  • DOI: 10.1016/j.ab.2011.11.010

Enriching pathogen transcripts from infected samples: A capture-based approach to enhanced host–pathogen RNA sequencing
journal, July 2013
  • Bent, Zachary W.; Tran-Gyamfi, Mary B.; Langevin, Stanley A.
  • Analytical Biochemistry, Vol. 438, Issue 1, p. 90-96
  • DOI: 10.1016/j.ab.2013.03.008

On-line monitoring system for chemical warfare agents using automated capillary micellar electrokinetic chromatography
journal, August 2012
  • VanderNoot, Victoria; Ferko, Scott; Van De Vreugde, James
  • Journal of Chromatography A, Vol. 1249, p. 233-240
  • DOI: 10.1016/j.chroma.2012.06.010