skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Multiplexed microfluidic approach for nucleic acid enrichment

Abstract

A system for enhancing a nucleic acid sample may include a one pump, a denaturing chamber; a microfluidic hydroxyapatite chromatography device configured for performing hydroxyapatite chromatography on the nucleic acid sample, a sample collector, and tubing connecting the pump with the denaturing chamber, the hydroxyapatite chromatography device and the sample collector such that the pump may be used to move the nucleic acid sample from the denaturing chamber to the hydroxyapatite chromatography device and then to the sample collector.

Inventors:
; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1248895
Patent Number(s):
9,322,014
Application Number:
14/084,937
Assignee:
Sandia Corporation (Albuquerque, NM)
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Nov 20
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

VanderNoot, Victoria A., Langevin, Stanley Alan, Bent, Zachary, Renzi, Ronald F., Ferko, Scott M., Van De Vreugde, James L., Lane, Todd, Patel, Kamlesh, and Branda, Steven. Multiplexed microfluidic approach for nucleic acid enrichment. United States: N. p., 2016. Web.
VanderNoot, Victoria A., Langevin, Stanley Alan, Bent, Zachary, Renzi, Ronald F., Ferko, Scott M., Van De Vreugde, James L., Lane, Todd, Patel, Kamlesh, & Branda, Steven. Multiplexed microfluidic approach for nucleic acid enrichment. United States.
VanderNoot, Victoria A., Langevin, Stanley Alan, Bent, Zachary, Renzi, Ronald F., Ferko, Scott M., Van De Vreugde, James L., Lane, Todd, Patel, Kamlesh, and Branda, Steven. Tue . "Multiplexed microfluidic approach for nucleic acid enrichment". United States. https://www.osti.gov/servlets/purl/1248895.
@article{osti_1248895,
title = {Multiplexed microfluidic approach for nucleic acid enrichment},
author = {VanderNoot, Victoria A. and Langevin, Stanley Alan and Bent, Zachary and Renzi, Ronald F. and Ferko, Scott M. and Van De Vreugde, James L. and Lane, Todd and Patel, Kamlesh and Branda, Steven},
abstractNote = {A system for enhancing a nucleic acid sample may include a one pump, a denaturing chamber; a microfluidic hydroxyapatite chromatography device configured for performing hydroxyapatite chromatography on the nucleic acid sample, a sample collector, and tubing connecting the pump with the denaturing chamber, the hydroxyapatite chromatography device and the sample collector such that the pump may be used to move the nucleic acid sample from the denaturing chamber to the hydroxyapatite chromatography device and then to the sample collector.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {4}
}

Patent:

Save / Share:

Works referenced in this record:

Generation of Multimillion-Sequence 16S rRNA Gene Libraries from Complex Microbial Communities by Assembling Paired-End Illumina Reads
journal, April 2011

  • Bartram, Andrea K.; Lynch, Michael D. J.; Stearns, Jennifer C.
  • Applied and Environmental Microbiology, Vol. 77, Issue 11
  • DOI: 10.1128/AEM.02772-10

Comparative Analysis of Human Protein-Coding and Noncoding RNAs between Brain and 10 Mixed Cell Lines by RNA-Seq
journal, November 2011


Size Exclusion Chromatography of Microliter Volumes for On-Line Use in Low-Pressure Microfluidic Systems
journal, August 2006

  • Chirica, G.; Lachmann, J.; Chan, J.
  • Analytical Chemistry, Vol. 78, Issue 15
  • DOI: 10.1021/ac060258t

Exome-wide DNA capture and next generation sequencing in domestic and wild species
journal, July 2011


Uncovering the Complexity of Transcriptomes with RNA-Seq
journal, January 2010

  • Costa, Valerio; Angelini, Claudia; De Feis, Italia
  • Journal of Biomedicine and Biotechnology, Vol. 2010
  • DOI: 10.1155/2010/853916

Rare target enrichment for ultrasensitive PCR detection using cot–rehybridization and duplex-specific nuclease
journal, February 2012

  • Gijavanekar, Charul; Strych, Ulrich; Fofanov, Yuriy
  • Analytical Biochemistry, Vol. 421, Issue 1, p. 81-85
  • DOI: 10.1016/j.ab.2011.11.010

Automated analysis of mouse serum peptidome using restricted access media and nanoliquid chromatography–tandem mass spectrometry
journal, May 2011


An ‘equalized cDNA library’ by the reassociation of short double-stranded cDNAs
journal, January 1990


RNA sequencing: advances, challenges and opportunities
journal, December 2010

  • Ozsolak, Fatih; Milos, Patrice M.
  • Nature Reviews Genetics, Vol. 12, Issue 2
  • DOI: 10.1038/nrg2934

Review Application of RNA-seq to reveal the transcript profile in bacteria
journal, January 2011

  • Pinto, A. C.; Melo-Barbosa, H. P.; Miyoshi, A.
  • Genetics and Molecular Research, Vol. 10, Issue 3
  • DOI: 10.4238/vol10-3gmr1554

Identification of novel transcripts in annotated genomes using RNA-Seq
journal, June 2011


Construction and characterization of a normalized cDNA library.
journal, September 1994

  • Soares, M. B.; Bonaldo, M. F.; Jelene, P.
  • Proceedings of the National Academy of Sciences, Vol. 91, Issue 20
  • DOI: 10.1073/pnas.91.20.9228

Autonomous Microfluidic Sample Preparation System for Protein Profile-Based Detection of Aerosolized Bacterial Cells and Spores
journal, August 2007

  • Stachowiak, Jeanne C.; Shugard, Erin E.; Mosier, Bruce P.
  • Analytical Chemistry, Vol. 79, Issue 15
  • DOI: 10.1021/ac070567z

Hydroxyapatite-Mediated Separation of Double-Stranded DNA, Single-Stranded DNA, and RNA Genomes from Natural Viral Assemblages
journal, June 2010

  • Andrews-Pfannkoch, C.; Fadrosh, D. W.; Thorpe, J.
  • Applied and Environmental Microbiology, Vol. 76, Issue 15
  • DOI: 10.1128/AEM.00204-10

Enriching pathogen transcripts from infected samples: A capture-based approach to enhanced host–pathogen RNA sequencing
journal, July 2013

  • Bent, Zachary W.; Tran-Gyamfi, Mary B.; Langevin, Stanley A.
  • Analytical Biochemistry, Vol. 438, Issue 1, p. 90-96
  • DOI: 10.1016/j.ab.2013.03.008

Normalizing cDNA Libraries
journal, April 2010

  • Bogdanova, Ekaterina A.; Shagina, Irina; Barsova, Ekaterina V.
  • Current Protocols in Molecular Biology, Vol. 90, Issue 1
  • DOI: 10.1002/0471142727.mb0512s90

Repeated Sequences in DNA
journal, August 1968


Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
journal, January 2011

  • Fadrosh, Douglas W.; Andrews-Pfannkoch, Cynthia; Williamson, Shannon J.
  • Journal of Visualized Experiments, Issue 55
  • DOI: 10.3791/3146

Isolation and purification of recombinant proteins, antibodies and plasmid DNA with hydroxyapatite chromatography
journal, December 2011


Microchip separations of protein biotoxins using an integrated hand-held device
journal, March 2005

  • Fruetel, Julia A.; Renzi, Ronald F.; VanderNoot, Victoria A.
  • ELECTROPHORESIS, Vol. 26, Issue 6
  • DOI: 10.1002/elps.200406194

Comprehensive comparative analysis of strand-specific RNA sequencing methods
journal, August 2010

  • Levin, Joshua Z.; Yassour, Moran; Adiconis, Xian
  • Nature Methods, Vol. 7, Issue 9
  • DOI: 10.1038/nmeth.1491

cDNA selection: efficient PCR approach for the selection of cDNAs encoded in large chromosomal DNA fragments.
journal, November 1991

  • Parimoo, S.; Patanjali, S. R.; Shukla, H.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 21
  • DOI: 10.1073/pnas.88.21.9623

Construction of a uniform-abundance (normalized) cDNA library.
journal, March 1991

  • Patanjali, S. R.; Parimoo, S.; Weissman, S. M.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 5
  • DOI: 10.1073/pnas.88.5.1943

Hand-Held Microanalytical Instrument for Chip-Based Electrophoretic Separations of Proteins
journal, January 2005

  • Renzi, Ronald F.; Stamps, James; Horn, Brent A.
  • Analytical Chemistry, Vol. 77, Issue 2
  • DOI: 10.1021/ac049214f

Modeling biominerals formed by apatites and DNA
journal, December 2013

  • Revilla-López, Guillermo; Casanovas, Jordi; Bertran, Oscar
  • Biointerphases, Vol. 8, Issue 1
  • DOI: 10.1186/1559-4106-8-10

Application for PCR technology to subtractive cDNA cloning: identification of genes expressed specifically in murine plasmacytoma cells
journal, January 1990

  • Timblin, Cynthia; Battey, James; Kuehl, W. Michael
  • Nucleic Acids Research, Vol. 18, Issue 6
  • DOI: 10.1093/nar/18.6.1587

On-line monitoring system for chemical warfare agents using automated capillary micellar electrokinetic chromatography
journal, August 2012

  • VanderNoot, Victoria; Ferko, Scott; Van De Vreugde, James
  • Journal of Chromatography A, Vol. 1249, p. 233-240
  • DOI: 10.1016/j.chroma.2012.06.010

populacDNA normalization by hydroxyapatite chromatography to enrich transcriptome diversity in RNA-seq applications
journal, December 2012

  • VanderNoot, Victoria A.; Langevin, Stanley A.; Solberg, Owen D.
  • BioTechniques, Vol. 53, Issue 6
  • DOI: 10.2144/000113937

Development of an integrated microfluidic instrument for unattended water-monitoring applications
journal, July 2010

  • VanderNoot, Victoria A.; Renzi, Ronald F.; Mosier, Bruce P.
  • ELECTROPHORESIS, Vol. 31, Issue 15
  • DOI: 10.1002/elps.201000052