Title: PNNL DataHub Project: Omics Lethal Human Viruses Project Profiling of the Host Response to Ebola Virus Infection, Processed Experimental Dataset Catalog

Abstract

Ebola virus (EBOV) is high risk biological agent, classified as a Category A priority pathogen (Flaviviridae) by the National Institute of Allergy and Infectious Diseases (NIAID), known to cause hemorrhagic fever with high mortality rates in humans. Lethal host-pathogen invasion mechanisms and the cellular intricacies behind these fatal infections still remain unclear. The NIAID Modeling Host Responses to Understand Severe Human Virus Infections Research Program project (2013-2018) aimed to develop an improved comprehensive understanding of the host response to a suite of viruses causing lethal infections leveraging a systems biology approach. Herein, PNNL sub-projects provide a never before released comprehensive infectious disease collection of primary and secondary transformation multi-Omics data profiling a series of priority pathogen primary experimental studies for enhanced open-access to viral Omics datasets and project lifecycle metadata. Secondary host-pathogen viral dataset downloads contain one or more statistically processed (normalization data transformation)  quantitative dataset collections resulting in qualitative expression analyses of primary host-pathogen experimental study designs. Leveraging unique high-resolution Omics capabilities for proteomics (P), metabolomics (M), lipidomics (L), and transcriptomics (T) dataset downloads each have a direct relationship to a primary sample submission corresponding to a specific Ebola virus [NCBITAXON:186536] (Zaire/Makona or Zaire/Mayinga) experimental infection study. Human host samples types include peripheral blood mononuclear cellsmore » isolated from blood plasma ["PBMC", BTO:0001025], human hepatoma carcinoma cells ["HUH", BTO:0001950], human umbilical vein endothelial cells ["HUVEC", BTO:0001949], immortalized human hepatocyte cells ["IHH", BTO:0006147], and human histiocytic lymphoma cells ["U937", BTO:0001412]. « less

Authors:

Anderson, Lindsey N. ;

• Search DOE Data Explorer for author "Anderson, Lindsey N."
• Search DOE Data Explorer for ORCID "0000-0002-8741-7823"

Eisfeld, Amie J. ;

• Search DOE Data Explorer for author "Eisfeld, Amie J."
• Search DOE Data Explorer for ORCID "0000-0003-2231-3107"

Waters, Katrina M.

• Search DOE Data Explorer for author "Waters, Katrina M."
• Search DOE Data Explorer for ORCID "0000-0003-4696-5396"

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+ Show Author Affiliations

1. Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
2. Univ. of Wisconsin, Madison, WI (United States). School of Veterinary Medicine. Pathology Department

Publication Date:

Mon Jan 18 00:00:00 EST 2021

Other Number(s):

LHVEBOV
EH001;EHUH001;EHUH002;EHUH003;EHUVEC001;EIHH001;EIHH002;EU937001

DOE Contract Number:  

AC05-76RL01830

Research Org.:

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:

USDOE Office of Science (SC); National Institute of Allergy and Infectious Diseases (NIAID)

Collaborations:

Modeling Host Responses to Understand Severe Human Virus Infections Program Project

Subject:

59 BASIC BIOLOGICAL SCIENCES

Keywords:

Virology [edam.topic:0781]; Immune Response [GO:0006955]; Human virus [NCIT:C14317]; Ebolavirus [NCBITAXON:186536]; Time Sampled Measurement Datasets [IAO:0000584]; Mass spectrometry data [edam.data:2536]; MassIVE dataset identifier [MS:1002487]; Gene expression profile data [edam.data:0928]; BioProject Accession Number [NCIT:C175890]; Omics [edam.topic:3391]; Multi-omics [edam.topic:4021]; data transformation [SIO:000594]; differential expression analysis [SWO:7000018]; Dataset Collection [schema:DataCatalog]

OSTI Identifier:

1784282

DOI:

https://doi.org/10.25584/LHVEBOV/1784282