Long-range barcode labeling-sequencing
Abstract
Methods for sequencing single large DNA molecules by clonal multiple displacement amplification using barcoded primers. Sequences are binned based on barcode sequences and sequenced using a microdroplet-based method for sequencing large polynucleotide templates to enable assembly of haplotype-resolved complex genomes and metagenomes.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1329319
- Patent Number(s):
- 9469874
- Application Number:
- 13/653,343
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Oct 16
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Chen, Feng, Zhang, Tao, Singh, Kanwar K., Pennacchio, Len A., Froula, Jeff L., and Eng, Kevin S. Long-range barcode labeling-sequencing. United States: N. p., 2016.
Web.
Chen, Feng, Zhang, Tao, Singh, Kanwar K., Pennacchio, Len A., Froula, Jeff L., & Eng, Kevin S. Long-range barcode labeling-sequencing. United States.
Chen, Feng, Zhang, Tao, Singh, Kanwar K., Pennacchio, Len A., Froula, Jeff L., and Eng, Kevin S. Tue .
"Long-range barcode labeling-sequencing". United States. https://www.osti.gov/servlets/purl/1329319.
@article{osti_1329319,
title = {Long-range barcode labeling-sequencing},
author = {Chen, Feng and Zhang, Tao and Singh, Kanwar K. and Pennacchio, Len A. and Froula, Jeff L. and Eng, Kevin S.},
abstractNote = {Methods for sequencing single large DNA molecules by clonal multiple displacement amplification using barcoded primers. Sequences are binned based on barcode sequences and sequenced using a microdroplet-based method for sequencing large polynucleotide templates to enable assembly of haplotype-resolved complex genomes and metagenomes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 18 00:00:00 EDT 2016},
month = {Tue Oct 18 00:00:00 EDT 2016}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Comprehensive human genome amplification using multiple displacement amplification
journal, April 2002
- Dean, F. B.; Hosono, S.; Fang, L.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 8, p. 5261-5266