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Title: Method for rapid base sequencing in DNA and RNA with two base labeling

Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.
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  1. (Los Alamos, NM)
Issue Date:
OSTI Identifier:
Regents of University of California Office of Technology Transfer (Alameda, CA) LANL
Patent Number(s):
US 5405747
Contract Number:
Research Org:
Los Alamos National Laboratory (LANL), Los Alamos, NM
Country of Publication:
United States
method; rapid; base; sequencing; dna; rna; labeling; rapid-base; two-base; employing; fluorescent; detection; single; molecules; wavelengths; bases; modified; accept; labels; replicate; strand; sequenced; sequentially; cleaved; replicated; excited; chosen; spectrum; electromagnetic; radiation; fluorescence; individual; tagged; detected; cleavage; electromagnetic radiation; rapid base; base sequencing; fluorescent labels; single molecule; fluorescent detection; /435/436/