Method for rapid base sequencing in DNA and RNA with two base labeling
Abstract
Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.
- Inventors:
-
- Los Alamos, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- OSTI Identifier:
- 869830
- Patent Number(s):
- 5405747
- Assignee:
- Regents of University of California Office of Technology Transfer (Alameda, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; rapid; base; sequencing; dna; rna; labeling; rapid-base; two-base; employing; fluorescent; detection; single; molecules; wavelengths; bases; modified; accept; labels; replicate; strand; sequenced; sequentially; cleaved; replicated; excited; chosen; spectrum; electromagnetic; radiation; fluorescence; individual; tagged; detected; cleavage; electromagnetic radiation; rapid base; base sequencing; fluorescent labels; single molecule; fluorescent detection; /435/436/
Citation Formats
Jett, James H, Keller, Richard A, Martin, John C, Posner, Richard G, Marrone, Babetta L, Hammond, Mark L, and Simpson, Daniel J. Method for rapid base sequencing in DNA and RNA with two base labeling. United States: N. p., 1995.
Web.
Jett, James H, Keller, Richard A, Martin, John C, Posner, Richard G, Marrone, Babetta L, Hammond, Mark L, & Simpson, Daniel J. Method for rapid base sequencing in DNA and RNA with two base labeling. United States.
Jett, James H, Keller, Richard A, Martin, John C, Posner, Richard G, Marrone, Babetta L, Hammond, Mark L, and Simpson, Daniel J. Sun .
"Method for rapid base sequencing in DNA and RNA with two base labeling". United States. https://www.osti.gov/servlets/purl/869830.
@article{osti_869830,
title = {Method for rapid base sequencing in DNA and RNA with two base labeling},
author = {Jett, James H and Keller, Richard A and Martin, John C and Posner, Richard G and Marrone, Babetta L and Hammond, Mark L and Simpson, Daniel J},
abstractNote = {Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1995},
month = {Sun Jan 01 00:00:00 EST 1995}
}
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Works referenced in this record:
Fluorescence detection in automated DNA sequence analysis
journal, June 1986
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A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides
journal, October 1987
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Single-molecule detection as an approach to rapid DNA sequencing
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High-Speed DNA Sequencing: An Approach Based Upon Fluorescence Detection of Single Molecules
journal, October 1989
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