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Title: Method for rapid base sequencing in DNA and RNA with two base labeling

Abstract

Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.

Inventors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Los Alamos, NM
Issue Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
OSTI Identifier:
869830
Patent Number(s):
5405747
Assignee:
Regents of University of California Office of Technology Transfer (Alameda, CA)
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; rapid; base; sequencing; dna; rna; labeling; rapid-base; two-base; employing; fluorescent; detection; single; molecules; wavelengths; bases; modified; accept; labels; replicate; strand; sequenced; sequentially; cleaved; replicated; excited; chosen; spectrum; electromagnetic; radiation; fluorescence; individual; tagged; detected; cleavage; electromagnetic radiation; rapid base; base sequencing; fluorescent labels; single molecule; fluorescent detection; /435/436/

Citation Formats

Jett, James H, Keller, Richard A, Martin, John C, Posner, Richard G, Marrone, Babetta L, Hammond, Mark L, and Simpson, Daniel J. Method for rapid base sequencing in DNA and RNA with two base labeling. United States: N. p., 1995. Web.
Jett, James H, Keller, Richard A, Martin, John C, Posner, Richard G, Marrone, Babetta L, Hammond, Mark L, & Simpson, Daniel J. Method for rapid base sequencing in DNA and RNA with two base labeling. United States.
Jett, James H, Keller, Richard A, Martin, John C, Posner, Richard G, Marrone, Babetta L, Hammond, Mark L, and Simpson, Daniel J. Sun . "Method for rapid base sequencing in DNA and RNA with two base labeling". United States. https://www.osti.gov/servlets/purl/869830.
@article{osti_869830,
title = {Method for rapid base sequencing in DNA and RNA with two base labeling},
author = {Jett, James H and Keller, Richard A and Martin, John C and Posner, Richard G and Marrone, Babetta L and Hammond, Mark L and Simpson, Daniel J},
abstractNote = {Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

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