skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores

Abstract

Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.

Inventors:
 [1];  [2];  [3];  [4]
  1. (Orinda, CA)
  2. (Moraga, CA)
  3. (Richmond, CA)
  4. (Redwood City, CA)
Issue Date:
Research Org.:
University of California
OSTI Identifier:
873390
Patent Number(s):
6150107
Assignee:
Regents of University of California (Oakland, CA) OAK
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
methods; sequencing; detection; energy; transfer; labels; cyanine; dyes; donor; chromophores; fluorophore; light; absorbed; transferred; acceptor; responds; emitting; fluorescent; impart; unusually; sensitivity; improving; usefulness; wide; variety; biochemical; procedures; particularly; nucleic; acid; fragment; sizing; related; cyanine dyes; light energy; fluorescent light; energy transfer; nucleic acid; wide variety; acid sequencing; transfer labels; donor fluorophore; fragment sizing; chemical procedures; chemical procedure; acid fragment; donor chromophores; /435/999/

Citation Formats

Glazer, Alexander N., Mathies, Richard A., Hung, Su-Chun, and Ju, Jingyue. Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores. United States: N. p., 2000. Web.
Glazer, Alexander N., Mathies, Richard A., Hung, Su-Chun, & Ju, Jingyue. Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores. United States.
Glazer, Alexander N., Mathies, Richard A., Hung, Su-Chun, and Ju, Jingyue. Sat . "Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores". United States. https://www.osti.gov/servlets/purl/873390.
@article{osti_873390,
title = {Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores},
author = {Glazer, Alexander N. and Mathies, Richard A. and Hung, Su-Chun and Ju, Jingyue},
abstractNote = {Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}

Patent:

Save / Share: