Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores
Abstract
Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.
- Inventors:
-
- Orinda, CA
- Moraga, CA
- Richmond, CA
- Redwood City, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 873390
- Patent Number(s):
- 6150107
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG03-91ER61125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- methods; sequencing; detection; energy; transfer; labels; cyanine; dyes; donor; chromophores; fluorophore; light; absorbed; transferred; acceptor; responds; emitting; fluorescent; impart; unusually; sensitivity; improving; usefulness; wide; variety; biochemical; procedures; particularly; nucleic; acid; fragment; sizing; related; cyanine dyes; light energy; fluorescent light; energy transfer; nucleic acid; wide variety; acid sequencing; transfer labels; donor fluorophore; fragment sizing; chemical procedures; chemical procedure; acid fragment; donor chromophores; /435/999/
Citation Formats
Glazer, Alexander N, Mathies, Richard A, Hung, Su-Chun, and Ju, Jingyue. Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores. United States: N. p., 2000.
Web.
Glazer, Alexander N, Mathies, Richard A, Hung, Su-Chun, & Ju, Jingyue. Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores. United States.
Glazer, Alexander N, Mathies, Richard A, Hung, Su-Chun, and Ju, Jingyue. Sat .
"Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores". United States. https://www.osti.gov/servlets/purl/873390.
@article{osti_873390,
title = {Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores},
author = {Glazer, Alexander N and Mathies, Richard A and Hung, Su-Chun and Ju, Jingyue},
abstractNote = {Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {1}
}
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Works referenced in this record:
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