Polynucleotide barcoding
Abstract
A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Illinois at Urbana-Champaign, IL (United States); Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1531658
- Patent Number(s):
- 7829278
- Application Number:
- 10/976,546
- Assignee:
- Board of Trustees of the University of Illinois (Urbana, IL); Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-91ER45439; T32-GM08276; DBI-02-15869; MCB-99-84841CAR
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2004-10-28
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Selvin, Paul R., Gordon, Matthew, Kwok, Pui -Yan, Xiao, Ming, and Chan, Ting -Fung. Polynucleotide barcoding. United States: N. p., 2010.
Web.
Selvin, Paul R., Gordon, Matthew, Kwok, Pui -Yan, Xiao, Ming, & Chan, Ting -Fung. Polynucleotide barcoding. United States.
Selvin, Paul R., Gordon, Matthew, Kwok, Pui -Yan, Xiao, Ming, and Chan, Ting -Fung. Tue .
"Polynucleotide barcoding". United States. https://www.osti.gov/servlets/purl/1531658.
@article{osti_1531658,
title = {Polynucleotide barcoding},
author = {Selvin, Paul R. and Gordon, Matthew and Kwok, Pui -Yan and Xiao, Ming and Chan, Ting -Fung},
abstractNote = {A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is determined to form a barcode representation of the polynucleotide wherein the targets and their relative positions are represented.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {11}
}
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Works referenced in this record:
Padlock probes: circularizing oligonucleotides for localized DNA detection
journal, September 1994
- Nilsson, M.; Malmgren, H.; Samiotaki, M.
- Science, Vol. 265, Issue 5181, p. 2085-2088
Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
patent, January 2000
- Söderlund, Hans; Syvanen, Anne-Christine
- US Patent Document 6,013,431
Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
journal, June 2003
- Yildiz, A.
- Science, Vol. 300, Issue 5628