Multiple tag labeling method for DNA sequencing
Abstract
A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.
- Inventors:
-
- Contra Costa County, CA
- Mt. View, CA
- San Francisco, CA
- Issue Date:
- Research Org.:
- Univ. of California (United States)
- OSTI Identifier:
- 870001
- Patent Number(s):
- 5436130
- Assignee:
- Regents of University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG03-91ER61125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- multiple; tag; labeling; method; dna; sequencing; described; single; lane; channel; electrophoresis; fragments; separated; detected; laser-excited; confocal; fluorescence; scanner; set; distinguished; binary; coding; scheme; employing; fluorescent; labels; radio-isotope; dna sequencing; fluorescent labels; fluorescence scanner; sequencing method; coding scheme; /435/436/
Citation Formats
Mathies, Richard A, Huang, Xiaohua C, and Quesada, Mark A. Multiple tag labeling method for DNA sequencing. United States: N. p., 1995.
Web.
Mathies, Richard A, Huang, Xiaohua C, & Quesada, Mark A. Multiple tag labeling method for DNA sequencing. United States.
Mathies, Richard A, Huang, Xiaohua C, and Quesada, Mark A. Sun .
"Multiple tag labeling method for DNA sequencing". United States. https://www.osti.gov/servlets/purl/870001.
@article{osti_870001,
title = {Multiple tag labeling method for DNA sequencing},
author = {Mathies, Richard A and Huang, Xiaohua C and Quesada, Mark A},
abstractNote = {A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}
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