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Title: Multiple tag labeling method for DNA sequencing

Abstract

A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.

Inventors:
 [1];  [2];  [3]
  1. (Contra Costa County, CA)
  2. (Mt. View, CA)
  3. (San Francisco, CA)
Issue Date:
Research Org.:
University of California
OSTI Identifier:
870001
Patent Number(s):
5436130
Assignee:
Regents of University of California (Oakland, CA) OAK
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
multiple; tag; labeling; method; dna; sequencing; described; single; lane; channel; electrophoresis; fragments; separated; detected; laser-excited; confocal; fluorescence; scanner; set; distinguished; binary; coding; scheme; employing; fluorescent; labels; radio-isotope; dna sequencing; fluorescent labels; fluorescence scanner; sequencing method; coding scheme; /435/436/

Citation Formats

Mathies, Richard A., Huang, Xiaohua C., and Quesada, Mark A. Multiple tag labeling method for DNA sequencing. United States: N. p., 1995. Web.
Mathies, Richard A., Huang, Xiaohua C., & Quesada, Mark A. Multiple tag labeling method for DNA sequencing. United States.
Mathies, Richard A., Huang, Xiaohua C., and Quesada, Mark A. Sun . "Multiple tag labeling method for DNA sequencing". United States. https://www.osti.gov/servlets/purl/870001.
@article{osti_870001,
title = {Multiple tag labeling method for DNA sequencing},
author = {Mathies, Richard A. and Huang, Xiaohua C. and Quesada, Mark A.},
abstractNote = {A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {1}
}

Patent:

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