Methods for determining enzymatic activity comprising heating and agitation of closed volumes
Abstract
Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
- Inventors:
- Issue Date:
- Research Org.:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1243062
- Patent Number(s):
- 9284596
- Application Number:
- 14/180,161
- Assignee:
- Battelle Energy Alliance, LLC (Idaho Falls, ID)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC07-05ID14517
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 Feb 13
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Thompson, David Neil, Henriksen, Emily DeCrescenzo, Reed, David William, and Jensen, Jill Renee. Methods for determining enzymatic activity comprising heating and agitation of closed volumes. United States: N. p., 2016.
Web.
Thompson, David Neil, Henriksen, Emily DeCrescenzo, Reed, David William, & Jensen, Jill Renee. Methods for determining enzymatic activity comprising heating and agitation of closed volumes. United States.
Thompson, David Neil, Henriksen, Emily DeCrescenzo, Reed, David William, and Jensen, Jill Renee. Tue .
"Methods for determining enzymatic activity comprising heating and agitation of closed volumes". United States. https://www.osti.gov/servlets/purl/1243062.
@article{osti_1243062,
title = {Methods for determining enzymatic activity comprising heating and agitation of closed volumes},
author = {Thompson, David Neil and Henriksen, Emily DeCrescenzo and Reed, David William and Jensen, Jill Renee},
abstractNote = {Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 15 00:00:00 EDT 2016},
month = {Tue Mar 15 00:00:00 EDT 2016}
}
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