Engineered enzymatically active bacteriophages and methods of uses thereof

Collins, James J; Kobayashi, Hideki; Kearn, Mads; Araki, Michihiro; Friedland, Ari; Lu, Timothy Kuan-Ta

Title: Engineered enzymatically active bacteriophages and methods of uses thereof

Patent · Tue May 22 00:00:00 EDT 2012

OSTI ID:1042632

Collins, James J ^[1]; Kobayashi, Hideki ^[2]; Kearn, Mads ^[3]; Araki, Michihiro ^[4]; Friedland, Ari ^[5]; Lu, Timothy Kuan-Ta ^[6]

Newton, MA
Yokohama, JP
Ottawa, CA
Minatoku, JP
Boston, MA
Palo Alto, CA

The present invention provides engineered bacteriophages that express at least one biofilm degrading enzyme on their surface and uses thereof for degrading bacterial biofilms. The invention also provides genetically engineered bacteriophages expressing the biofilm degrading enzymes and proteins necessary for the phage to replicate in different naturally occurring biofilm producing bacteria. The phages of the invention allow a method of biofilm degradation by the use of one or only a few administration of the phage because the system using these phages is self perpetuating, and capable of degrading biofilm even when the concentration of bacteria within the biofilm is low.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

Assignee:: Trustees of Boston University (Boston, MA); Massachusetts Institute of Technology (Cambridge, MA)

Patent Number(s):: 8,182,804

Application Number:: 11/662,551

OSTI ID:: 1042632

Country of Publication:: United States

Language:: English

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