Engineered enzymatically active bacteriophages and methods of uses thereof
Abstract
The present invention provides engineered bacteriophages that express at least one biofilm degrading enzyme on their surface and uses thereof for degrading bacterial biofilms. The invention also provides genetically engineered bacteriophages expressing the biofilm degrading enzymes and proteins necessary for the phage to replicate in different naturally occurring biofilm producing bacteria. The phages of the invention allow a method of biofilm degradation by the use of one or only a few administration of the phage because the system using these phages is self perpetuating, and capable of degrading biofilm even when the concentration of bacteria within the biofilm is low.
- Inventors:
-
- Newton, MA
- Yokohama, JP
- Ottawa, CA
- Minatoku, JP
- Boston, MA
- Palo Alto, CA
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1042632
- Patent Number(s):
- 8182804
- Application Number:
- 11/662,551
- Assignee:
- Trustees of Boston University (Boston, MA); Massachusetts Institute of Technology (Cambridge, MA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61L - METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Collins, James J, Kobayashi, Hideki, Kearn, Mads, Araki, Michihiro, Friedland, Ari, and Lu, Timothy Kuan-Ta. Engineered enzymatically active bacteriophages and methods of uses thereof. United States: N. p., 2012.
Web.
Collins, James J, Kobayashi, Hideki, Kearn, Mads, Araki, Michihiro, Friedland, Ari, & Lu, Timothy Kuan-Ta. Engineered enzymatically active bacteriophages and methods of uses thereof. United States.
Collins, James J, Kobayashi, Hideki, Kearn, Mads, Araki, Michihiro, Friedland, Ari, and Lu, Timothy Kuan-Ta. Tue .
"Engineered enzymatically active bacteriophages and methods of uses thereof". United States. https://www.osti.gov/servlets/purl/1042632.
@article{osti_1042632,
title = {Engineered enzymatically active bacteriophages and methods of uses thereof},
author = {Collins, James J and Kobayashi, Hideki and Kearn, Mads and Araki, Michihiro and Friedland, Ari and Lu, Timothy Kuan-Ta},
abstractNote = {The present invention provides engineered bacteriophages that express at least one biofilm degrading enzyme on their surface and uses thereof for degrading bacterial biofilms. The invention also provides genetically engineered bacteriophages expressing the biofilm degrading enzymes and proteins necessary for the phage to replicate in different naturally occurring biofilm producing bacteria. The phages of the invention allow a method of biofilm degradation by the use of one or only a few administration of the phage because the system using these phages is self perpetuating, and capable of degrading biofilm even when the concentration of bacteria within the biofilm is low.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 22 00:00:00 EDT 2012},
month = {Tue May 22 00:00:00 EDT 2012}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Bacteriophage Typing of the Staphylococci
journal, January 1963
- Wentworth, Berttina B.
- Bacteriological Reviews, Vol. 27, Issue 3
Long-circulating bacteriophage as antibacterial agents.
journal, April 1996
- Merril, C. R.; Biswas, B.; Carlton, R.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 8
Therapy of Experimental Pseudomonas Infections with a Nonreplicating Genetically Modified Phage
journal, October 2004
- Hagens, Steven; Habel, André; von Ahsen, Uwe
- Antimicrobial Agents and Chemotherapy, Vol. 48, Issue 10
Tropism switching in Bordetella bacteriophage defines a family of diversity-generating retroelements
journal, September 2004
- Doulatov, Sergei; Hodes, Asher; Dai, Lixin
- Nature, Vol. 431, Issue 7007
Bacteriophage and associated polysaccharide depolymerases – novel tools for study of bacterial biofilms
journal, September 1998
- Hughes, K. A.; Sutherland, I. W.; Clark, J.
- Journal of Applied Microbiology, Vol. 85, Issue 3
Refactoring bacteriophage T7
journal, January 2005
- Chan, Leon Y.; Kosuri, Sriram; Endy, Drew
- Molecular Systems Biology, Vol. 1, Issue 1
The prospect for bacteriophage therapy in Western medicine
journal, June 2003
- Merril, Carl R.; Scholl, Dean; Adhya, Sankar L.
- Nature Reviews Drug Discovery, Vol. 2, Issue 6
Tight Modulation of Escherichia coli Bacterial Biofilm Formation through Controlled Expression of Adhesion Factors
journal, March 2007
- Da Re, S.; Le Quere, B.; Ghigo, J. -M.
- Applied and Environmental Microbiology, Vol. 73, Issue 10
Lytic infection of Escherichia coli biofilms by bacteriophage T4
journal, January 1995
- Doolittle, M. M.; Cooney, J. J.; Caldwell, D. E.
- Canadian Journal of Microbiology, Vol. 41, Issue 1
A linguistic model for the rational design of antimicrobial peptides
journal, October 2006
- Loose, Christopher; Jensen, Kyle; Rigoutsos, Isidore
- Nature, Vol. 443, Issue 7113
Natural conjugative plasmids induce bacterial biofilm development
journal, July 2001
- Ghigo, Jean-Marc
- Nature, Vol. 412, Issue 6845
Treatment of Clostridium difficile-associated disease: old therapies and new strategies
journal, September 2005
- Aslam, Saima; Hamill, Richard J.; Musher, Daniel M.
- The Lancet Infectious Diseases, Vol. 5, Issue 9
Microbial Biofilms
journal, October 1995
- Costerton, J. W.; Lewandowski, Z.; Caldwell, D. E.
- Annual Review of Microbiology, Vol. 49, Issue 1
Antibiotic resistance of bacteria in biofilms
journal, July 2001
- Stewart, Philip S.; William Costerton, J.
- The Lancet, Vol. 358, Issue 9276
Incomplete entry of bacteriophage T7 DNA into F plasmid-containing Escherichia coli.
journal, January 1995
- García, L. R.; Molineux, I. J.
- Journal of bacteriology, Vol. 177, Issue 14
Escherichia coli K1's Capsule Is a Barrier to Bacteriophage T7
journal, August 2005
- Scholl, Dean; Adhya, Sankar; Merril, Carl
- Applied and Environmental Microbiology, Vol. 71, Issue 8
Bacteriophage Therapy
journal, October 2001
- Summers, William C.
- Annual Review of Microbiology, Vol. 55, Issue 1
The superficial life of microbes
journal, May 2006
- Kolter, Roberto; Greenberg, E. Peter
- Nature, Vol. 441, Issue 7091
Bacterial Biofilms: An Emerging Link to Disease Pathogenesis
journal, October 2003
- Parsek, Matthew R.; Singh, Pradeep K.
- Annual Review of Microbiology, Vol. 57, Issue 1
Synthetic biology: new engineering rules for an emerging discipline
journal, January 2006
- Andrianantoandro, Ernesto; Basu, Subhayu; Karig, David K.
- Molecular Systems Biology, Vol. 2, Issue 1
Environmentally Controlled Invasion of Cancer Cells by Engineered Bacteria
journal, January 2006
- Anderson, J. Christopher; Clarke, Elizabeth J.; Arkin, Adam P.
- Journal of Molecular Biology, Vol. 355, Issue 4
Organization and Expression of Bacteriophage T7 DNA
journal, January 1983
- Studier, F. W.; Dunn, J. J.
- Cold Spring Harbor Symposia on Quantitative Biology, Vol. 47, Issue 0
Using Bacteriophages To Reduce Formation of Catheter-Associated Biofilms by Staphylococcus epidermidis
journal, April 2006
- Curtin, John J.; Donlan, Rodney M.
- Antimicrobial Agents and Chemotherapy, Vol. 50, Issue 4
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
journal, June 1983
- Dunn, John J.; Studier, F. William; Gottesman, M.
- Journal of Molecular Biology, Vol. 166, Issue 4, p. 477-535
Biofilm-control strategies based on enzymic disruption of the extracellular polymeric substance matrix – a modelling study
journal, December 2005
- Xavier, Joao B.; Picioreanu, Cristian; Rani, Suriani Abdul
- Microbiology, Vol. 151, Issue 12
Bacteriophage T4 multiplication in a glucose-limited Escherichia coli biofilm
journal, July 2001
- Corbin, Brian D.; McLean, Robert JC; Aron, Gary M.
- Canadian Journal of Microbiology, Vol. 47, Issue 7
Narrative Review: The New Epidemic of Clostridium difficile –Associated Enteric Disease
journal, November 2006
- Bartlett, John G.
- Annals of Internal Medicine, Vol. 145, Issue 10
Engineering Life: Building a FAB for Biology
journal, June 2006
- Baker, David; Church, George; Collins, Jim
- Scientific American, Vol. 294, Issue 6
Tracing the interaction of bacteriophage with bacterial biofilms using fluorescent and chromogenic probes
journal, June 1996
- Doolittle, M. M.; Cooney, J. J.; Caldwell, D. E.
- Journal of Industrial Microbiology, Vol. 16, Issue 6
Phage-inspired antibiotics?
journal, February 2004
- Projan, Steven
- Nature Biotechnology, Vol. 22, Issue 2
Phage offer a real alternative
journal, May 2004
- Schoolnik, Gary K.; Summers, William C.; Watson, James D.
- Nature Biotechnology, Vol. 22, Issue 5
The Calgary Biofilm Device: New Technology for Rapid Determination of Antibiotic Susceptibilities of Bacterial Biofilms
journal, January 1999
- Ceri, H.; Olson, M. E.; Stremick, C.
- Journal of Clinical Microbiology, Vol. 37, Issue 6
Biofilm susceptibility to bacteriophage attack: the role of phage-borne polysaccharide depolymerase
journal, November 1998
- Hughes, K. A.; Sutherland, I. W.; Jones, M. V.
- Microbiology, Vol. 144, Issue 11
Microbial Biofilms: from Ecology to Molecular Genetics
journal, December 2000
- Davey, M. E.; O'toole, G. A.
- Microbiology and Molecular Biology Reviews, Vol. 64, Issue 4
From The Cover: Combining two genomes in one cell: Stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome
journal, October 2005
- Itaya, M.; Tsuge, K.; Koizumi, M.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 44
Engineered gene circuits
journal, November 2002
- Hasty, Jeff; McMillen, David; Collins, J. J.
- Nature, Vol. 420, Issue 6912
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000
- Datsenko, K. A.; Wanner, B. L.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
Production of the antimalarial drug precursor artemisinic acid in engineered yeast
journal, April 2006
- Ro, Dae-Kyun; Paradise, Eric M.; Ouellet, Mario
- Nature, Vol. 440, Issue 7086, p. 940-943
Polysaccharases for microbial exopolysaccharides
journal, April 1999
- Sutherland, Ian W.
- Carbohydrate Polymers, Vol. 38, Issue 4
Bacterial Biofilms: A Common Cause of Persistent Infections
journal, May 1999
- Costerton, J. W.
- Science, Vol. 284, Issue 5418, p. 1318-1322
Aminoglycoside antibiotics induce bacterial biofilm formation
journal, August 2005
- Hoffman, Lucas R.; D'Argenio, David A.; MacCoss, Michael J.
- Nature, Vol. 436, Issue 7054
Extracellular DNA Required for Bacterial Biofilm Formation
journal, February 2002
- Whitchurch, C. B.
- Science, Vol. 295, Issue 5559
Defective transcription of the right end of bacteriophage T7 DNA during an abortive infection of F plasmid-containing Escherichia coli.
journal, January 1991
- Beck, P. J.; Molineux, I. J.
- Journal of Bacteriology, Vol. 173, Issue 3
Depolymerization of β-1,6-N-Acetyl-d-Glucosamine Disrupts the Integrity of Diverse Bacterial Biofilms
journal, January 2005
- Itoh, Yoshikane; Wang, Xin; Hinnebusch, B. Joseph
- Journal of Bacteriology, Vol. 187, Issue 1
Phage Therapy in Terms of Bacteriophage Genetics: Hopes, Prospects, Safety, Limitations
journal, July 2001
- Krylov, V. N.
- Russian Journal of Genetics, Vol. 37, Issue 7, p. 715-730
Phage typing of Salmonella enteritidis in the United States.
journal, January 1991
- Hickman-Brenner, F. W.; Stubbs, A. D.; Farmer, J. J.
- Journal of Clinical Microbiology, Vol. 29, Issue 12
Accurate multiplex gene synthesis from programmable DNA microchips
journal, December 2004
- Tian, Jingdong; Gong, Hui; Sheng, Nijing
- Nature, Vol. 432, Issue 7020, p. 1050-1054
Reverse Transcriptase-Mediated Tropism Switching in Bordetella Bacteriophage
journal, March 2002
- Liu, M.
- Science, Vol. 295, Issue 5562