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Title: Engineered enzymatically active bacteriophages and methods of uses thereof

Abstract

The present invention provides engineered bacteriophages that express at least one biofilm degrading enzyme on their surface and uses thereof for degrading bacterial biofilms. The invention also provides genetically engineered bacteriophages expressing the biofilm degrading enzymes and proteins necessary for the phage to replicate in different naturally occurring biofilm producing bacteria. The phages of the invention allow a method of biofilm degradation by the use of one or only a few administration of the phage because the system using these phages is self perpetuating, and capable of degrading biofilm even when the concentration of bacteria within the biofilm is low.

Inventors:
 [1];  [2];  [3];  [4];  [5];  [6]
  1. Newton, MA
  2. Yokohama, JP
  3. Ottawa, CA
  4. Minatoku, JP
  5. Boston, MA
  6. Palo Alto, CA
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1042632
Patent Number(s):
8182804
Application Number:
11/662,551
Assignee:
Trustees of Boston University (Boston, MA); Massachusetts Institute of Technology (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Collins, James J, Kobayashi, Hideki, Kearn, Mads, Araki, Michihiro, Friedland, Ari, and Lu, Timothy Kuan-Ta. Engineered enzymatically active bacteriophages and methods of uses thereof. United States: N. p., 2012. Web.
Collins, James J, Kobayashi, Hideki, Kearn, Mads, Araki, Michihiro, Friedland, Ari, & Lu, Timothy Kuan-Ta. Engineered enzymatically active bacteriophages and methods of uses thereof. United States.
Collins, James J, Kobayashi, Hideki, Kearn, Mads, Araki, Michihiro, Friedland, Ari, and Lu, Timothy Kuan-Ta. Tue . "Engineered enzymatically active bacteriophages and methods of uses thereof". United States. https://www.osti.gov/servlets/purl/1042632.
@article{osti_1042632,
title = {Engineered enzymatically active bacteriophages and methods of uses thereof},
author = {Collins, James J and Kobayashi, Hideki and Kearn, Mads and Araki, Michihiro and Friedland, Ari and Lu, Timothy Kuan-Ta},
abstractNote = {The present invention provides engineered bacteriophages that express at least one biofilm degrading enzyme on their surface and uses thereof for degrading bacterial biofilms. The invention also provides genetically engineered bacteriophages expressing the biofilm degrading enzymes and proteins necessary for the phage to replicate in different naturally occurring biofilm producing bacteria. The phages of the invention allow a method of biofilm degradation by the use of one or only a few administration of the phage because the system using these phages is self perpetuating, and capable of degrading biofilm even when the concentration of bacteria within the biofilm is low.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {5}
}

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