Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Sommer, Gregory J; Hatch, Anson V; Singh, Anup K; Wang, Ying-Chih

Title: Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Patent · Tue Oct 28 00:00:00 EDT 2014

OSTI ID:1162116

Sommer, Gregory J; Hatch, Anson V; Singh, Anup K; Wang, Ying-Chih

Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.

View Patent

Cite

Export

Save

Research Organization:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia Corporation (Albuquerque, NM)

Patent Number(s):: 8,871,496

Application Number:: 12/544,982

OSTI ID:: 1162116

Resource Relation:: Patent File Date: 2009 Aug 20

Country of Publication:: United States

Language:: English

References (47)

Simultaneous particle separation and chemical reaction Wu, Caicai; Weigl, Bernhard; Kenny, Margaret https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6221677 US Patent Document 6,221,677	patent	April 2001
Microfabricated devices and method of manufacturing the same Beebe, David J.; Moore, Jeffrey S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6488872 US Patent Document 6,488,872	patent	December 2002
Classification of lung carcinomas using gene expression analysis Golub, Todd R.; Meyerson, Matthew; Bhattacharjee, Arindam https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040009489 US Patent Application 10/259233; 20040009489	patent-application	January 2004
Microfluidic analytic detection assays, devices, and integrated systems Wada, H. Garrett; Murphy, Matthew B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040048360 US Patent Application 10/613220; 20040048360	patent-application	March 2004
Axial Nonuniformities and Flow in Columns for Capillary Electrochromatography Rathore, A. S.; Horváth, Cs. Analytical Chemistry, Vol. 70, Issue 14 https://doi.org/10.1021/ac971260a	journal	July 1998
Dynamic Labeling during Capillary or Microchip Electrophoresis for Laser-Induced Fluorescence Detection of Protein−SDS Complexes without Pre- or Postcolumn Labeling Jin, Lian Ji; Giordano, Braden C.; Landers, James P. Analytical Chemistry, Vol. 73, Issue 20 https://doi.org/10.1021/ac010590z	journal	October 2001
Sample Filtration, Concentration, and Separation Integrated on Microfluidic Devices Broyles, B. Scott; Jacobson, Stephen C.; Ramsey, J. Michael Analytical Chemistry, Vol. 75, Issue 11 https://doi.org/10.1021/ac025503x	journal	June 2003
Micro Total Analysis Systems. Recent Developments Vilkner, Torsten; Janasek, Dirk; Manz, Andreas Analytical Chemistry, Vol. 76, Issue 12 https://doi.org/10.1021/ac040063q	journal	June 2004
Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J. Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984 https://doi.org/10.1021/ac0600454	journal	July 2006
Monolithic Porous Polymer for On-Chip Solid-Phase Extraction and Preconcentration Prepared by Photoinitiated in Situ Polymerization within a Microfluidic Device Yu, Cong; Davey, Mark H.; Svec, Frantisek Analytical Chemistry, Vol. 73, Issue 21 https://doi.org/10.1021/ac0106288	journal	November 2001
Ligand Exchange. II.¹ Separation of Ligands Having Different Coördinative Valences Helfferich, F. Journal of the American Chemical Society, Vol. 84, Issue 17, p. 3242-3245 https://doi.org/10.1021/ja00876a004	journal	September 1962
Microfluidic immunoassays as rapid saliva-based clinical diagnostics Herr, A. E.; Hatch, A. V.; Throckmorton, D. J. Proceedings of the National Academy of Sciences, Vol. 104, Issue 13, p. 5268-5273 https://doi.org/10.1073/pnas.0607254104	journal	March 2007
Measurement of the Porous Microstructure of Hydrogels by Nuclear Magnetic Resonance Chui, Martin M.; Phillips, Ronald J.; McCarthy, Michael J. Journal of Colloid and Interface Science, Vol. 174, Issue 2 https://doi.org/10.1006/jcis.1995.1399	journal	September 1995
Rapid protein separations in ultra-short microchannels: microchip sodium dodecyl sulfate–polyacrylamide gel electrophoresis and isoelectric focusing Han, J.; Singh, A. Journal of Chromatography A, Vol. 1049, Issue 1-2 https://doi.org/10.1016/S0021-9673(04)01346-9	journal	September 2004
Microfabricated Porous Membrane Structure for Sample Concentration and Electrophoretic Analysis Khandurina, Julia; Jacobson, Stephen C.; Waters, Larry C. Analytical Chemistry, Vol. 71, Issue 9 https://doi.org/10.1021/ac981161c	journal	May 1999
Electrophoretic Separation of Proteins on a Microchip with Noncovalent, Postcolumn Labeling Liu, Yingjie; Foote, Robert S.; Jacobson, Stephen C. Analytical Chemistry, Vol. 72, Issue 19 https://doi.org/10.1021/ac000625f	journal	October 2000
Micro Total Analysis Systems. 1. Introduction, Theory, and Technology Reyes, Darwin R.; Iossifidis, Dimitri; Auroux, Pierre-Alain Analytical Chemistry, Vol. 74, Issue 12 https://doi.org/10.1021/ac0202435	journal	June 2002
Micro Total Analysis Systems. 2. Analytical Standard Operations and Applications Auroux, Pierre-Alain; Iossifidis, Dimitri; Reyes, Darwin R. Analytical Chemistry, Vol. 74, Issue 12 https://doi.org/10.1021/ac020239t	journal	June 2002
Electrochromatography in Microchips: Reversed-Phase Separation of Peptides and Amino Acids Using Photopatterned Rigid Polymer Monoliths Throckmorton, Daniel J.; Shepodd, Timothy J.; Singh, Anup K. Analytical Chemistry, Vol. 74, Issue 4 https://doi.org/10.1021/ac011077o	journal	February 2002
Photopolymerized Cross-Linked Polyacrylamide Gels for On-Chip Protein Sizing Herr, Amy E.; Singh, Anup K. Analytical Chemistry, Vol. 76, Issue 16 https://doi.org/10.1021/ac049686u	journal	August 2004
Microchip Dialysis of Proteins Using in Situ Photopatterned Nanoporous Polymer Membranes Song, Simon; Singh, Anup K.; Shepodd, Timothy J. Analytical Chemistry, Vol. 76, Issue 8 https://doi.org/10.1021/ac035290r	journal	April 2004
Electrophoretic Concentration of Proteins at Laser-Patterned Nanoporous Membranes in Microchips Song, Simon; Singh, Anup K.; Kirby, Brian J. Analytical Chemistry, Vol. 76, Issue 15 https://doi.org/10.1021/ac0497151	journal	August 2004
On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V. Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333 https://doi.org/10.1021/ac702523g	journal	May 2008
3D polymeric microfluidic device fabrication via contact liquid photolithographic polymerization (CLiPP) Haraldsson, K. Tommy; Hutchison, J. Brian; Sebra, Robert P. Sensors and Actuators B: Chemical, Vol. 113, Issue 1, p. 454-460 https://doi.org/10.1016/j.snb.2005.03.096	journal	January 2006
Bio-particle separation using microfluidic porous plug for environmental monitoring Chon, Kyongmi; Moon, Jihee; Kim, Suhan Desalination, Vol. 202, Issue 1-3, p. 215-223 https://doi.org/10.1016/j.desal.2005.12.057	journal	January 2007
A capillary electrophoresis method to assay catalytic activity of botulinum neurotoxin serotypes: Implications for substrate specificity Purcell, Angela L.; Hoard-Fruchey, Heidi M. Analytical Biochemistry, Vol. 366, Issue 2, p. 207-217 https://doi.org/10.1016/j.ab.2007.04.048	journal	July 2007
Substrate Recognition of VAMP-2 by Botulinum Neurotoxin B and Tetanus Neurotoxin Chen, Sheng; Hall, Cherisse; Barbieri, Joseph T. Journal of Biological Chemistry, Vol. 283, Issue 30 https://doi.org/10.1074/jbc.M800611200	journal	May 2008
Initiation and kinetics of thiol-ene photopolymerizations without photoinitiators Cramer, Neil B.; Reddy, Sirish K.; Cole, Michael Journal of Polymer Science Part A: Polymer Chemistry, Vol. 42, Issue 22 https://doi.org/10.1002/pola.20419	journal	January 2004
Robust polymer microfluidic device fabrication via contact liquid photolithographic polymerization (CLiPP) Hutchison, J. Brian; Haraldsson, K. Tommy; Good, Brian T. Lab on a Chip, Vol. 4, Issue 6 https://doi.org/10.1039/b405985a	journal	January 2004
Parallel isoelectric focusing II Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651 https://doi.org/10.1002/elps.200406117	journal	November 2004
Microfluidic tectonics platform: A colorimetric, disposable botulinum toxin enzyme-linked immunosorbent assay system Moorthy, Jaisree; Mensing, Glennys A.; Kim, Dongshin ELECTROPHORESIS, Vol. 25, Issue 1011 https://doi.org/10.1002/elps.200405888	journal	June 2004
A continuous-flow instrument for the determination of linear polyacrylamide stability Klepárník, Karel; Mikuška, Pavel ELECTROPHORESIS, Vol. 25, Issue 14 https://doi.org/10.1002/elps.200035921	journal	July 2004
Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer Lim, Peniel; North, Robert; Vigh, Gyula ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859 https://doi.org/10.1002/elps.200600846	journal	June 2007
Detecting Single Stranded DNA with a Solid State Nanopore Fologea, Daniel; Gershow, Marc; Ledden, Bradley Nano Letters, Vol. 5, Issue 10, p. 1905-1909 https://doi.org/10.1021/nl051199m	journal	October 2005
Multiple separations of DNA sequencing fragments with a non-cross-linked polyacrylamide-filled capillary: capillary electrophoresis at 300 V/cm Figeys, Daniel; Dovichi, Norman J. Journal of Chromatography A, Vol. 717, Issue 1-2, p. 113-116 https://doi.org/10.1016/0021-9673(95)00649-2	journal	November 1995
Microfluidic tectonics: A comprehensive construction platform for microfluidic systems Beebe, D. J.; Moore, J. S.; Yu, Q. Proceedings of the National Academy of Sciences, Vol. 97, Issue 25, p. 13488-13493 https://doi.org/10.1073/pnas.250273097	journal	November 2000
Detection in capillary electrophoresis Swinney, Kelly; Bornhop, Darryl J. Electrophoresis, Vol. 21, Issue 7, p. 1239-1250 https://doi.org/10.1002/(SICI)1522-2683(20000401)21:7<1239::AID-ELPS1239>3.0.CO;2-6	journal	April 2000
Electrophoresis in microfabricated devices using photopolymerized polyacrylamide gels and electrode-defined sample injection Brahmasandra, Sundaresh N.; Ugaz, Victor M.; Burke, David T. ELECTROPHORESIS, Vol. 22, Issue 2 https://doi.org/10.1002/1522-2683(200101)22:2<300::AID-ELPS300>3.0.CO;2-F	journal	January 2001
Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques Cabrera, Catherine R.; Yager, Paul ELECTROPHORESIS, Vol. 22, Issue 2 https://doi.org/10.1002/1522-2683(200101)22:2<355::AID-ELPS355>3.0.CO;2-C	journal	January 2001
Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient Huang, Tiemin; Pawliszyn, Janusz ELECTROPHORESIS, Vol. 23, Issue 20, p. 3504-3510 https://doi.org/10.1002/1522-2683(200210)23:20<3504::AID-ELPS3504>3.0.CO;2-X	journal	October 2002
An integrated solid-phase extraction system for sub-picomolar detection Jemere, Abebaw Belay; Oleschuk, Richard D.; Ouchen, Fahima ELECTROPHORESIS, Vol. 23, Issue 20 https://doi.org/10.1002/1522-2683(200210)23:20<3537::AID-ELPS3537>3.0.CO;2-9	journal	October 2002
Ultra rapid prototyping of microfluidic systems using liquid phase photopolymerization Khoury, Christopher; Mensing, Glennys A.; Beebe, David J. Lab on a Chip, Vol. 2, Issue 1 https://doi.org/10.1039/b109344d	journal	January 2002
Preparative-scale isoelectric trapping separations using a modified Gradiflow unit Ogle, David; Ho, Agnes; Gibson, Toby Journal of Chromatography A, Vol. 979, Issue 1-2, p. 155-161 https://doi.org/10.1016/S0021-9673(02)01601-1	journal	December 2002
Sample pre-concentration by isotachophoresis in microfluidic devices Wainright, Ann; Williams, Stephen J.; Ciambrone, Gary Journal of Chromatography A, Vol. 979, Issue 1-2, p. 69-80 https://doi.org/10.1016/S0021-9673(02)01246-3	journal	December 2002
Parallel processing in the isoelectric focusing chip Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744 https://doi.org/10.1002/elps.200305663	journal	November 2003
Formation of a resistive region at the anode end in DNA capillary electrophoresis Bilenko, Olga; Gavrilov, Dmitri; Gorbovitski, Boris ELECTROPHORESIS, Vol. 24, Issue 78 https://doi.org/10.1002/elps.200390151	journal	April 2003
Dynamic analyte introduction and focusing in plastic microfluidic devices for proteomic analysis Li, Yan; DeVoe, Donald L.; Lee, Cheng S. ELECTROPHORESIS, Vol. 24, Issue 12 https://doi.org/10.1002/elps.200390013	journal	January 2003

Similar Records

A Single-Domain Llama Antibody Potently Inhibits the Enzymatic Activity of Botulinum Neurotoxin by Binding to the Non-Catalytic [alpha]-Exosite Binding Region

Journal Article · Fri Aug 13 00:00:00 EDT 2010 · J. Mol. Biol. · OSTI ID:1162116

Dong, Jianbo; Thompson, Aaron A; Fan, Yongfeng; +7 more

DHS Summer Student Project Report

Technical Report · Fri Aug 19 00:00:00 EDT 2005 · OSTI ID:1162116

Kawamoto, S

Bead-Based Assays for Biodetection: From Flow-Cytometry to Microfluidics

Conference · Mon May 04 00:00:00 EDT 2009 · OSTI ID:1162116

Ozanich, Richard M; Antolick, Kathryn C; Bruckner-Lea, Cindy J; +5 more

Related Subjects

60 APPLIED LIFE SCIENCES

Title: Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Citation Formats

References (47)

Similar Records

Related Subjects