Global to push GA events into
skip to main content
  • DOepatents
  • Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Title: Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1162116
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
Patent Number(s):
8,871,496
Application Number:
12/544,982
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2009 Aug 20
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES
[ × clear filter / sort ]

Works referenced in this record:

Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels
journal, July 2006
  • Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J.
  • Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984
  • DOI: 10.1021/ac0600454
Ligand Exchange. II.1 Separation of Ligands Having Different Coördinative Valences
journal, September 1962
  • Helfferich, F.
  • Journal of the American Chemical Society, Vol. 84, Issue 17, p. 3242-3245
  • DOI: 10.1021/ja00876a004
Microfluidic immunoassays as rapid saliva-based clinical diagnostics
journal, March 2007
  • Herr, A. E.; Hatch, A. V.; Throckmorton, D. J.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 13, p. 5268-5273
  • DOI: 10.1073/pnas.0607254104
On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients
journal, May 2008
  • Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V.
  • Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333
  • DOI: 10.1021/ac702523g
3D polymeric microfluidic device fabrication via contact liquid photolithographic polymerization (CLiPP)
journal, January 2006
  • Haraldsson, K. Tommy; Hutchison, J. Brian; Sebra, Robert P.
  • Sensors and Actuators B: Chemical, Vol. 113, Issue 1, p. 454-460
  • DOI: 10.1016/j.snb.2005.03.096
Bio-particle separation using microfluidic porous plug for environmental monitoring
journal, January 2007
A capillary electrophoresis method to assay catalytic activity of botulinum neurotoxin serotypes: Implications for substrate specificity
journal, July 2007
  • Purcell, Angela L.; Hoard-Fruchey, Heidi M.
  • Analytical Biochemistry, Vol. 366, Issue 2, p. 207-217
  • DOI: 10.1016/j.ab.2007.04.048
Parallel isoelectric focusing II
journal, November 2004
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651
  • DOI: 10.1002/elps.200406117
Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer
journal, June 2007
  • Lim, Peniel; North, Robert; Vigh, Gyula
  • ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859
  • DOI: 10.1002/elps.200600846
Detecting Single Stranded DNA with a Solid State Nanopore
journal, October 2005
  • Fologea, Daniel; Gershow, Marc; Ledden, Bradley
  • Nano Letters, Vol. 5, Issue 10, p. 1905-1909
  • DOI: 10.1021/nl051199m
Multiple separations of DNA sequencing fragments with a non-cross-linked polyacrylamide-filled capillary: capillary electrophoresis at 300 V/cm
journal, November 1995
Microfluidic tectonics: A comprehensive construction platform for microfluidic systems
journal, November 2000
  • Beebe, D. J.; Moore, J. S.; Yu, Q.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 25, p. 13488-13493
  • DOI: 10.1073/pnas.250273097
Detection in capillary electrophoresis
journal, April 2000
Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient
journal, October 2002
Preparative-scale isoelectric trapping separations using a modified Gradiflow unit
journal, December 2002
Sample pre-concentration by isotachophoresis in microfluidic devices
journal, December 2002
  • Wainright, Ann; Williams, Stephen J.; Ciambrone, Gary
  • Journal of Chromatography A, Vol. 979, Issue 1-2, p. 69-80
  • DOI: 10.1016/S0021-9673(02)01246-3
Parallel processing in the isoelectric focusing chip
journal, November 2003
  • Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel
  • ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744
  • DOI: 10.1002/elps.200305663

    Similar records in DOepatents and OSTI.GOV collections: