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Title: Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Abstract

Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.

Inventors:
; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1162116
Patent Number(s):
8871496
Application Number:
12/544,982
Assignee:
Sandia Corporation (Albuquerque, NM)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2009 Aug 20
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Sommer, Gregory J, Hatch, Anson V, Singh, Anup K, and Wang, Ying-Chih. Methods, microfluidic devices, and systems for detection of an active enzymatic agent. United States: N. p., 2014. Web.
Sommer, Gregory J, Hatch, Anson V, Singh, Anup K, & Wang, Ying-Chih. Methods, microfluidic devices, and systems for detection of an active enzymatic agent. United States.
Sommer, Gregory J, Hatch, Anson V, Singh, Anup K, and Wang, Ying-Chih. Tue . "Methods, microfluidic devices, and systems for detection of an active enzymatic agent". United States. https://www.osti.gov/servlets/purl/1162116.
@article{osti_1162116,
title = {Methods, microfluidic devices, and systems for detection of an active enzymatic agent},
author = {Sommer, Gregory J and Hatch, Anson V and Singh, Anup K and Wang, Ying-Chih},
abstractNote = {Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {10}
}

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