Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Title: Methods, microfluidic devices, and systems for detection of an active enzymatic agent

Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent.

Inventors:: Sommer, Gregory J; Hatch, Anson V; Singh, Anup K; Wang, Ying-Chih

Issue Date:: 2014-10-28

OSTI Identifier:: 1162116

Assignee:: Sandia Corporation (Albuquerque, NM) SNL-A

Patent Number(s):: 8,871,496

Application Number:: 12/544,982

Contract Number:: AC04-94AL85000

Resource Relation:: Patent File Date: 2009 Aug 20

Research Org:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES

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