Title: Recominant Pinoresino-Lariciresinol Reductase, Recombinant Dirigent Protein And Methods Of Use

Abstract

Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided from source species Forsythia intermedia, Thuja plicata, Tsuga heterophylla, Eucommia ulmoides, Linum usitatissimum, and Schisandra chinensis, which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.

Inventors:

Lewis, Norman G ^[1]; Davin, Laurence B ^[1]; Dinkova-Kostova, Albena T ^[2]; Fujita, Masayuki ^[3]; David R. ^[4]; Simo, Ford ^[5]; Joshua, D ^[1]

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+ Show Inventor Affiliations

1. Pullman, WA
2. Baltimore, MD
3. (Kita-gun, JP), Gang
4. (Ann Arbor, MI), Sarkanen
5. Minneapolis, MN

Issue Date:

Tue Oct 21 00:00:00 EDT 2003

Research Org.:

Washington State University Research Foundation (Pullman, WA)

OSTI Identifier:

879674

Patent Number(s):

6635459

Application Number:

09/704640

Assignee:

Washington State University Research Foundation (Pullman, WA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/415 - from plants

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/8243 - {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}
C12N9/0004 - {Oxidoreductases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P17/04 - containing a five-membered hetero ring, e.g. griseofulvin {, vitamin C}
C12P7/22 - aromatic

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DOE Contract Number:  

FG03-97ER20259

Resource Type:

Patent

Country of Publication:

United States

Language:

English