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Title: Systems and methods for the secretion of recombinant proteins in gram negative bacteria

Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1344502
Assignee:
WISCONSIN ALUMNI RESEARCH FOUNDATION CHO
Patent Number(s):
9,574,197
Application Number:
15/211,632
Contract Number:
FC02-07ER64494
Resource Relation:
Patent File Date: 2016 Jul 15
Research Org:
WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
journal, October 1989
  • Ward, E. Sally; G├╝ssow, Detlef; Griffiths, Andrew D.
  • Nature, Vol. 341, Issue 6242, p. 544-546
  • DOI: 10.1038/341544a0

A Protein Export Pathway Involving Escherichia coli Porins
journal, July 2012

Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli
journal, December 2005
  • Zhang, Guijin; Brokx, Stephen; Weiner, Joel H
  • Nature Biotechnology, Vol. 24, Issue 1, p. 100-104
  • DOI: 10.1038/nbt1174