RNA binding protein and binding site useful for expression of recombinant molecules
Abstract
The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.
- Inventors:
- Issue Date:
- Research Org.:
- Scripps Research Institute, San Diego, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1176706
- Patent Number(s):
- RE39350
- Application Number:
- 10/310,587
- Assignee:
- Scripps Research Institute
- DOE Contract Number:
- FG03-93ER20116
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Mayfield, Stephen P. RNA binding protein and binding site useful for expression of recombinant molecules. United States: N. p., 2006.
Web.
Mayfield, Stephen P. RNA binding protein and binding site useful for expression of recombinant molecules. United States.
Mayfield, Stephen P. Tue .
"RNA binding protein and binding site useful for expression of recombinant molecules". United States. https://www.osti.gov/servlets/purl/1176706.
@article{osti_1176706,
title = {RNA binding protein and binding site useful for expression of recombinant molecules},
author = {Mayfield, Stephen P.},
abstractNote = {The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {10}
}
Works referenced in this record:
Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.
journal, December 1991
- Danon, A.; Mayfield, S. P.
- The EMBO Journal, Vol. 10, Issue 13
Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum
journal, August 1990
- D�ring, Klaus; Hippe, Sigrun; Kreuzaler, Fritz
- Plant Molecular Biology, Vol. 15, Issue 2
ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation.
journal, May 1994
- Danon, A.; Mayfield, S. P.
- The EMBO Journal, Vol. 13, Issue 9