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Title: Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use

Patent ·
OSTI ID:873645
 [1];  [1];  [2];  [3];  [4];  [5];  [1]
  1. Pullman, WA
  2. Baltimore, MD
  3. Kagawa, JP
  4. Ann Arbor, MI
  5. S. Minneapolis, MN
+ Show Author Affiliations

Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.

Research Organization:
Washington State University Research Foundation (Pullman, Washington)
DOE Contract Number:
FG03-97ER20259
Assignee:
Washington State University Research Foundation (Pullman, Washington);
Patent Number(s):
US 6210942
Application Number:
09/475316
OSTI ID:
873645
Country of Publication:
United States
Language:
English

References (3)

Stereospecificity of (+)-pinoresinol and (+)-lariciresinol reductases from Forsythia intermedia. journal December 1993
Novel benzylic ether reductions in lignan biogenesis in Forsythia intermedia journal June 1993
An extraordinary accumulation of (−)-pinoresinol in cell-free extracts of Forsythia intermedia: evidence for enantiospecific reduction of (+)-pinoresinol journal January 1992

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recombinant
pinoresinol
lariciresinol
reductase
dirigent
protein
methods
proteins
reductases
isolated
cdnas
encoding
accordingly
dna
sequences
provided
code
expression
aspects
replicable
cloning
vehicles
base
sequence
sufficiently
complementary
portion
rna
enable
hybridization
therewith
modified
host
cells
transformed
transfected
infected
injected
vehicle
systems
sequence sufficiently
modified host
cloning vehicles
recombinant expression
replicable recombinant
enable hybridization
hybridization therewith
recombinant cloning
dna sequences
dna sequence
isolated dna
host cells
sequence encoding
base sequence
cloning vehicle
host cell
sufficiently complementary
cdnas encoding
dirigent proteins
lariciresinol reductase
dirigent protein
/435/999/