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Nucleic and amino acid sequences relating to a novel transketolase, and methods for the expression thereof

Patent ·
OSTI ID:873567
 [1];  [2];  [1];  [1]
  1. Pullman, WA
  2. Colfax, WA
+ Show Author Affiliations

cDNAs encoding 1-deoxyxylulose-5-phosphate synthase from peppermint (Mentha piperita) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7) are provided which code for the expression of 1-deoxyxylulose-5-phosphate synthase from plants. In another aspect the present invention provides for isolated, recombinant DXPS proteins, such as the proteins having the sequences set forth in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8. In other aspects, replicable recombinant cloning vehicles are provided which code for plant 1-deoxyxylulose-5-phosphate synthases, or for a base sequence sufficiently complementary to at least a portion of 1-deoxyxylulose-5-phosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a plant 1-deoxyxylulose-5-phosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant 1-deoxyxylulose-5-phosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant 1-deoxyxylulose-5-phosphate synthase may be used to obtain expression or enhanced expression of 1-deoxyxylulose-5-phosphate synthase in plants in order to enhance the production of 1-deoxyxylulose-5-phosphate, or its derivatives such as isopentenyl diphosphate (BP), or may be otherwise employed for the regulation or expression of 1-deoxyxylulose-5-phosphate synthase, or the production of its products.

Research Organization:
Washington State University Research Foundation (Pullman, WA)
DOE Contract Number:
FG03-96ER20212
Assignee:
Washington State University Research Foundation (Pullman, WA)
Patent Number(s):
US 6190895
OSTI ID:
873567
Country of Publication:
United States
Language:
English

References (9)

Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate journal October 1993
CLA1, a novel gene required for chloroplast development, is highly conserved in evolution journal May 1996
LESSONS FROM SEQUENCING OF THE GENOME OF A UNICELLULAR CYANOBACTERIUM, SYNECHOCYSTIS SP. PCC6803 journal June 1998
Is Isoprene Emitted by Plants Synthesized via the Novel Isopentenyl Pyrophosphate Pathway? journal February 1997
Examples of chemical modification and recombinant DNA approaches with hemoglobin journal January 1996
Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin. journal November 1981
A common structural motif in thiamin pyrophosphate-binding enzymes journal September 1989
Binary vectors which allow the exchange of plant selectable markers and reporter genes journal January 1990
Nucleotide and deduced polypeptide sequences of the photosynthetic reaction-center, B870 antenna, and flanking polypeptides from R. capsulata journal July 1984

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/435/999/
1-deoxyxylulose-5-phosphate
accordingly
acid
acid sequence
acid sequences
aforementioned
aforementioned recombinant
amino
amino acid
amounts
aspect
aspects
base
base sequence
bp
cdnas
cdnas encoding
cells
cloning
cloning vehicle
cloning vehicles
code
complementary
corresponding
corresponding amino
derivatives
determined
diphosphate
dna
dna sequence
dna sequences
dxps
employed
enable
enable hybridization
encoding
enhance
enhanced
enhanced expression
expression
facilitate
forth
host
host cell
host cells
hybridization
hybridization therewith
infected
injected
isolated
isolated dna
isolation
isopentenyl
mentha
mentha piperita
methods
modified
modified host
novel
nucleic
obtain
obtain expression
otherwise
otherwise employed
peppermint
piperita
plant
plants
portion
production
products
proteins
provided
provides
purification
recombinant
recombinant cloning
recombinant expression
regulation
relating
replicable
replicable recombinant
rna
seq
sequence
sequence encoding
sequence sufficiently
sequenced
sequences
set
set forth
significant
significant amount
significant amounts
sufficiently
sufficiently complementary
synthase
synthase dna
synthases
systems
therewith
transfected
transformed
transketolase
vehicle
vehicles