Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene
- Pullman, WA
- Colfax, WA
- Moscow, ID
A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.
- Research Organization:
- Washington State University Research Foundation (Pullman, WA)
- DOE Contract Number:
- FG03-96ER20212
- Assignee:
- Washington State University Research Foundation (Pullman, WA)
- Patent Number(s):
- US 6008043
- OSTI ID:
- 872786
- Country of Publication:
- United States
- Language:
- English
Purification and Characterization of Trans-β-farnesene Synthase from Maritime Pine (Pinus pinaster Ait.) Needles
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acid
acid sequence
aforementioned
aforementioned recombinant
alarm
amino
amino acid
amounts
aphid
aspects
bacterial
base
base sequence
beta
cdna
cdna clone
cdna encoding
cells
chi
clone
cloning
cloning vehicle
cloning vehicles
code
codes
complementary
corresponding
corresponding amino
determined
dna
dna encoding
dna sequence
employed
enable
enable hybridization
encoding
enhance
enhanced
enhanced expression
expression
facilitate
host
host cell
host cells
hybridization
hybridization therewith
infected
injected
isolated
isolated dna
isolation
mentha
mentha piperita
methods
modified
modified host
obtain
obtain expression
otherwise
otherwise employed
peppermint
pheromone
piperita
plants
portion
produces
product
production
provided
purification
recombinant
recombinant cloning
recombinant expression
regulation
replicable
replicable recombinant
rna
seq
sequence
sequence encoding
sequence sufficiently
sequenced
sesquiterpene
significant
significant amount
significant amounts
sufficiently
sufficiently complementary
synthase
synthase dna
systems
therewith
transfected
transformed
vehicle
vehicles