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Title: Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use

Abstract

A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Mentha x piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint (Mentha x piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or themore » production of its products.« less

Inventors:
 [1];  [1]
  1. (Pullman, WA)
Publication Date:
Research Org.:
Washington State University Research Foundation (Pullman, WA)
OSTI Identifier:
873752
Patent Number(s):
US 6235514
Assignee:
Washington State University Research Foundation (Pullman, WA) OAK
DOE Contract Number:  
FG03-96ER20212
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
nucleic; acid; molecules; encoding; isopentenyl; monophosphate; kinase; methods; cdna; ipk; peppermint; mentha; piperita; isolated; sequenced; corresponding; amino; sequence; determined; accordingly; dna; seq; provided; codes; expression; aspects; replicable; recombinant; cloning; vehicles; code; base; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; aforementioned; facilitate; production; isolation; purification; significant; amounts; obtain; enhanced; plants; enhance; isoprenoids; derived; therefrom; otherwise; employed; regulation; products; sequence sufficiently; acid molecule; modified host; cloning vehicles; recombinant expression; replicable recombinant; obtain expression; otherwise employed; enable hybridization; hybridization therewith; recombinant cloning; dna sequence; isolated dna; significant amounts; acid molecules; acid sequence; amino acid; nucleic acid; host cells; sequence encoding; base sequence; cloning vehicle; dna encoding; isopentenyl monophosphate; host cell; sufficiently complementary; monophosphate kinase; cdna encoding; derived therefrom; significant amount; corresponding amino; enhanced expression; aforementioned recombinant; encoding isopentenyl; /435/514/999/

Citation Formats

Croteau, Rodney B., and Lange, Bernd M. Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use. United States: N. p., 2001. Web.
Croteau, Rodney B., & Lange, Bernd M. Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use. United States.
Croteau, Rodney B., and Lange, Bernd M. Mon . "Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use". United States. https://www.osti.gov/servlets/purl/873752.
@article{osti_873752,
title = {Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use},
author = {Croteau, Rodney B. and Lange, Bernd M.},
abstractNote = {A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Mentha x piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint (Mentha x piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or the production of its products.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}

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