skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nucleic acid molecules encoding isopentenyl monophosphate kinase, and methods of use

Patent ·
OSTI ID:873752
 [1];  [1]
  1. Pullman, WA
+ Show Author Affiliations

A cDNA encoding isopentenyl monophosphate kinase (IPK) from peppermint (Mentha x piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of isopentenyl monophosphate kinase (SEQ ID NO:2), from peppermint (Mentha x piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for isopentenyl monophosphate kinase, or for a base sequence sufficiently complementary to at least a portion of isopentenyl monophosphate kinase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding isopentenyl monophosphate kinase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant isopentenyl monophosphate kinase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant isopentenyl monophosphate kinase may be used to obtain expression or enhanced expression of isopentenyl monophosphate kinase in plants in order to enhance the production of isopentenyl monophosphate kinase, or isoprenoids derived therefrom, or may be otherwise employed for the regulation or expression of isopentenyl monophosphate kinase, or the production of its products.

Research Organization:
Washington State University Research Foundation (Pullman, WA)
DOE Contract Number:
FG03-96ER20212
Assignee:
Washington State University Research Foundation (Pullman, WA)
Patent Number(s):
US 6235514
OSTI ID:
873752
Country of Publication:
United States
Language:
English

References (6)

A family of transketolases that directs isoprenoid biosynthesis via a mevalonate-independent pathway journal March 1998
Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis journal March 1998
Dedicated Roles of Plastid Transketolases during the Early Onset of Isoprenoid Biogenesis in Pepper Fruits 1 journal August 1998
Chromoplast development in ripening tomato fruit: identification of cDNAs for chromoplast-targeted proteins and characterization of a cDNA encoding a plastid-localized low-molecular-weight heat shock protein journal February 1997
Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol journal November 1997
Isopentenyl diphosphate biosynthesis via a mevalonate-independent pathway: Isopentenyl monophosphate kinase catalyzes the terminal enzymatic step journal November 1999

Similar Records

Nucleic and amino acid sequences relating to a novel transketolase, and methods for the expression thereof
Patent · Mon Jan 01 00:00:00 EST 2001 · OSTI ID:873752
+1 more
Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene
Patent · Fri Jan 01 00:00:00 EST 1999 · OSTI ID:873752
Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene
Patent · Tue Jan 25 00:00:00 EST 2005 · OSTI ID:873752

Related Subjects

nucleic
acid
molecules
encoding
isopentenyl
monophosphate
kinase
methods
cdna
ipk
peppermint
mentha
piperita
isolated
sequenced
corresponding
amino
sequence
determined
accordingly
dna
seq
provided
codes
expression
aspects
replicable
recombinant
cloning
vehicles
code
base
sufficiently
complementary
portion
rna
enable
hybridization
therewith
modified
host
cells
transformed
transfected
infected
injected
vehicle
systems
aforementioned
facilitate
production
isolation
purification
significant
amounts
obtain
enhanced
plants
enhance
isoprenoids
derived
therefrom
otherwise
employed
regulation
products
sequence sufficiently
acid molecule
modified host
cloning vehicles
recombinant expression
replicable recombinant
obtain expression
otherwise employed
enable hybridization
hybridization therewith
recombinant cloning
dna sequence
isolated dna
significant amounts
acid molecules
acid sequence
amino acid
nucleic acid
host cells
sequence encoding
base sequence
cloning vehicle
dna encoding
isopentenyl monophosphate
host cell
sufficiently complementary
monophosphate kinase
cdna encoding
derived therefrom
significant amount
corresponding amino
enhanced expression
aforementioned recombinant
encoding isopentenyl
/435/514/999/