Isolation and bacterial expression of a sesquiterpene synthase CDNA clone from peppermint(mentha .chi. piperita, L.) that produces the aphid alarm pheromone (E)-.beta.-farnesene
- Pullman, WA
- Colfax, WA
- Moscow, ID
A cDNA encoding (E)-.beta.-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-.beta.-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperita). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-.beta.-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-.beta.-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-.beta.-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-.beta.-farnesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-.beta.-farnesene synthase may be used to obtain expression or enhanced expression of (E)-.beta.-farnesene synthase in plants in order to enhance the production of (E)-.beta.-farnesene, or may be otherwise employed for the regulation or expression of (E)-.beta.-farnesene synthase, or the production of its product.
- Research Organization:
- Washington State University Research Foundation (Pullman, WA)
- DOE Contract Number:
- FG03-96ER20212
- Assignee:
- Washington State University Research Foundation (Pullman, WA)
- Patent Number(s):
- US 6008043
- OSTI ID:
- 872786
- Country of Publication:
- United States
- Language:
- English
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bacterial
expression
sesquiterpene
synthase
cdna
clone
peppermint
mentha
chi
piperita
produces
aphid
alarm
pheromone
beta
-farnesene
encoding
isolated
sequenced
corresponding
amino
acid
sequence
determined
accordingly
dna
seq
provided
codes
aspects
replicable
recombinant
cloning
vehicles
code
base
sufficiently
complementary
portion
rna
enable
hybridization
therewith
modified
host
cells
transformed
transfected
infected
injected
vehicle
systems
methods
aforementioned
facilitate
production
purification
significant
amounts
obtain
enhanced
plants
enhance
otherwise
employed
regulation
product
sequence sufficiently
modified host
cloning vehicles
cdna clone
recombinant expression
replicable recombinant
obtain expression
otherwise employed
enable hybridization
hybridization therewith
synthase dna
recombinant cloning
dna sequence
isolated dna
significant amounts
acid sequence
amino acid
host cells
sequence encoding
base sequence
cloning vehicle
dna encoding
host cell
sufficiently complementary
cdna encoding
significant amount
mentha piperita
corresponding amino
enhanced expression
aforementioned recombinant
/435/999/