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Title: Nucleic and amino acid sequences relating to a novel transketolase, and methods for the expression thereof

Patent ·
OSTI ID:873567
 [1];  [2];  [1];  [1]
  1. Pullman, WA
  2. Colfax, WA
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cDNAs encoding 1-deoxyxylulose-5-phosphate synthase from peppermint (Mentha piperita) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7) are provided which code for the expression of 1-deoxyxylulose-5-phosphate synthase from plants. In another aspect the present invention provides for isolated, recombinant DXPS proteins, such as the proteins having the sequences set forth in SEQ ID NO:4, SEQ ID NO:6 and SEQ ID NO:8. In other aspects, replicable recombinant cloning vehicles are provided which code for plant 1-deoxyxylulose-5-phosphate synthases, or for a base sequence sufficiently complementary to at least a portion of 1-deoxyxylulose-5-phosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a plant 1-deoxyxylulose-5-phosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant 1-deoxyxylulose-5-phosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant 1-deoxyxylulose-5-phosphate synthase may be used to obtain expression or enhanced expression of 1-deoxyxylulose-5-phosphate synthase in plants in order to enhance the production of 1-deoxyxylulose-5-phosphate, or its derivatives such as isopentenyl diphosphate (BP), or may be otherwise employed for the regulation or expression of 1-deoxyxylulose-5-phosphate synthase, or the production of its products.

Research Organization:
Washington State University Research Foundation (Pullman, WA)
DOE Contract Number:
FG03-96ER20212
Assignee:
Washington State University Research Foundation (Pullman, WA)
Patent Number(s):
US 6190895
OSTI ID:
873567
Country of Publication:
United States
Language:
English

References (9)

CLA1, a novel gene required for chloroplast development, is highly conserved in evolution journal May 1996
A common structural motif in thiamin pyrophosphate-binding enzymes journal September 1989
Binary vectors which allow the exchange of plant selectable markers and reporter genes journal January 1990
Examples of chemical modification and recombinant DNA approaches with hemoglobin journal January 1996
Is Isoprene Emitted by Plants Synthesized via the Novel Isopentenyl Pyrophosphate Pathway? journal February 1997
Nucleotide and deduced polypeptide sequences of the photosynthetic reaction-center, B870 antenna, and flanking polypeptides from R. capsulata journal July 1984
Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate journal October 1993
Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin. journal November 1981
LESSONS FROM SEQUENCING OF THE GENOME OF A UNICELLULAR CYANOBACTERIUM, SYNECHOCYSTIS SP. PCC6803 journal June 1998

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Related Subjects

nucleic
amino
acid
sequences
relating
novel
transketolase
methods
expression
cdnas
encoding
1-deoxyxylulose-5-phosphate
synthase
peppermint
mentha
piperita
isolated
sequenced
corresponding
determined
accordingly
dna
seq
provided
code
plants
aspect
provides
recombinant
dxps
proteins
set
forth
aspects
replicable
cloning
vehicles
plant
synthases
base
sequence
sufficiently
complementary
portion
rna
enable
hybridization
therewith
modified
host
cells
transformed
transfected
infected
injected
vehicle
systems
aforementioned
facilitate
production
isolation
purification
significant
amounts
obtain
enhanced
enhance
derivatives
isopentenyl
diphosphate
bp
otherwise
employed
regulation
products
sequence sufficiently
modified host
cloning vehicles
recombinant expression
replicable recombinant
obtain expression
otherwise employed
enable hybridization
hybridization therewith
synthase dna
recombinant cloning
dna sequences
dna sequence
isolated dna
significant amounts
acid sequence
amino acid
acid sequences
host cells
sequence encoding
base sequence
cloning vehicle
host cell
sufficiently complementary
set forth
cdnas encoding
significant amount
mentha piperita
corresponding amino
enhanced expression
aforementioned recombinant
/435/999/