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Title: Recominant Pinoresino-Lariciresinol Reductase, Recombinant Dirigent Protein And Methods Of Use

Abstract

Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided from source species Forsythia intermedia, Thuja plicata, Tsuga heterophylla, Eucommia ulmoides, Linum usitatissimum, and Schisandra chinensis, which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.

Inventors:
 [1];  [1];  [2];  [3];  [4];  [5];  [1]
  1. (Pullman, WA)
  2. (Baltimore, MD)
  3. (Kita-gun, JP), Gang
  4. (Ann Arbor, MI), Sarkanen
  5. (Minneapolis, MN), Ford
Publication Date:
Research Org.:
Washington State University Research Foundation (Pullman, WA)
OSTI Identifier:
879674
Patent Number(s):
US 6635459
Application Number:
09/704640
Assignee:
Washington State University Research Foundation (Pullman, WA) OAK
DOE Contract Number:  
FG03-97ER20259
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Lewis, Norman G., Davin, Laurence B., Dinkova-Kostova, Albena T., Fujita, Masayuki, David R., Simo, and Joshua D. Recominant Pinoresino-Lariciresinol Reductase, Recombinant Dirigent Protein And Methods Of Use. United States: N. p., 2003. Web.
Lewis, Norman G., Davin, Laurence B., Dinkova-Kostova, Albena T., Fujita, Masayuki, David R., Simo, & Joshua D. Recominant Pinoresino-Lariciresinol Reductase, Recombinant Dirigent Protein And Methods Of Use. United States.
Lewis, Norman G., Davin, Laurence B., Dinkova-Kostova, Albena T., Fujita, Masayuki, David R., Simo, and Joshua D. Tue . "Recominant Pinoresino-Lariciresinol Reductase, Recombinant Dirigent Protein And Methods Of Use". United States. https://www.osti.gov/servlets/purl/879674.
@article{osti_879674,
title = {Recominant Pinoresino-Lariciresinol Reductase, Recombinant Dirigent Protein And Methods Of Use},
author = {Lewis, Norman G. and Davin, Laurence B. and Dinkova-Kostova, Albena T. and Fujita, Masayuki and David R. and Simo and Joshua D.},
abstractNote = {Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided from source species Forsythia intermedia, Thuja plicata, Tsuga heterophylla, Eucommia ulmoides, Linum usitatissimum, and Schisandra chinensis, which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 21 00:00:00 EDT 2003},
month = {Tue Oct 21 00:00:00 EDT 2003}
}

Patent:

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