Recominant Pinoresino-Lariciresinol Reductase, Recombinant Dirigent Protein And Methods Of Use

Patent · Tue Oct 21 00:00:00 EDT 2003

OSTI ID:879674

Lewis, Norman G ^[1]; Davin, Laurence B ^[1]; Dinkova-Kostova, Albena T ^[2]; Simo, Ford ^[3]; Joshua, D ^[1]

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1. Pullman, WA
2. Baltimore, MD
3. Minneapolis, MN

Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided from source species Forsythia intermedia, Thuja plicata, Tsuga heterophylla, Eucommia ulmoides, Linum usitatissimum, and Schisandra chinensis, which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.

• Research Organization: Washington State University Research Foundation (Pullman, WA)
• DOE Contract Number: FG03-97ER20259
• Assignee: Washington State University Research Foundation (Pullman, WA)
• Patent Number(s): US 6635459
• Application Number: 09/704640
• OSTI ID: 879674
• Country of Publication: United States
• Language: English