skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Metal binding proteins, recombinant host cells and methods

Abstract

The present disclosure provides artificial heavy metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metals taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.

Inventors:
;
Issue Date:
Research Org.:
University of Georgia Research Foundation, Inc., Athens, GA (Unites States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174899
Patent Number(s):
6750042
Application Number:
09/977,137
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
FG02-99ER62865
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Summers, Anne O., and Caguiat, Jonathan J. Metal binding proteins, recombinant host cells and methods. United States: N. p., 2004. Web.
Summers, Anne O., & Caguiat, Jonathan J. Metal binding proteins, recombinant host cells and methods. United States.
Summers, Anne O., and Caguiat, Jonathan J. Tue . "Metal binding proteins, recombinant host cells and methods". United States. https://www.osti.gov/servlets/purl/1174899.
@article{osti_1174899,
title = {Metal binding proteins, recombinant host cells and methods},
author = {Summers, Anne O. and Caguiat, Jonathan J.},
abstractNote = {The present disclosure provides artificial heavy metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metals taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {6}
}

Patent:

Save / Share:

Works referenced in this record:

Model for mammalian metallothionein structure.
journal, March 1983


Crystal structure of Cd,Zn metallothionein
journal, February 1986


Activator-dependent preinduction binding of .sigma.-70 RNA polymerase at the metal-regulated mer promoter
journal, October 1990


Communication between the active sites in dimeric mercuric ion reductase: an alternating sites hypothesis for catalysis
journal, March 1991


Transition metals in control of gene expression
journal, August 1993


Ultrasensitivity and heavy-metal selectivity of the allosterically modulated MerR transcription complex.
journal, May 1990


The MerR metalloregulatory protein binds mercuric ion as a tricoordinate, metal-bridged dimer
journal, February 1990


Cd(II)-Responsive and Constitutive Mutants Implicate a Novel Domain in MerR
journal, June 1999


Bacterial metal-resistance proteins and their use in biosensors for the detection of bioavailable heavy metals
journal, April 2000


Repeated fed-batch operations for microbial detoxification of mercury using wild-type and recombinant mercury-resistant bacteria
journal, October 1998


A Fluorescent Zinc Probe Based on Metal-Induced Peptide Folding
journal, January 1996


C-Terminal cysteines of Tn501 mercuric ion reductase
journal, February 1992