Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use
Abstract
Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.
- Inventors:
-
- Pullman, WA
- Baltimore, MD
- Kagawa, JP
- Ann Arbor, MI
- S. Minneapolis, MN
- Issue Date:
- Research Org.:
- Washington State University Research Foundation (Pullman, Washington)
- OSTI Identifier:
- 873645
- Patent Number(s):
- 6210942
- Application Number:
- 09/475316
- Assignee:
- Washington State University Research Foundation (Pullman, Washington);
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-97ER20259
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- recombinant; pinoresinol; lariciresinol; reductase; dirigent; protein; methods; proteins; reductases; isolated; cdnas; encoding; accordingly; dna; sequences; provided; code; expression; aspects; replicable; cloning; vehicles; base; sequence; sufficiently; complementary; portion; rna; enable; hybridization; therewith; modified; host; cells; transformed; transfected; infected; injected; vehicle; systems; sequence sufficiently; modified host; cloning vehicles; recombinant expression; replicable recombinant; enable hybridization; hybridization therewith; recombinant cloning; dna sequences; dna sequence; isolated dna; host cells; sequence encoding; base sequence; cloning vehicle; host cell; sufficiently complementary; cdnas encoding; dirigent proteins; lariciresinol reductase; dirigent protein; /435/999/
Citation Formats
Lewis, Norman G, Davin, Laurence B, Dinkova-Kostova, Albena T, Fujita, Masayuki, Gang, David R, Sarkanen, Simo, and Ford, Joshua D. Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use. United States: N. p., 2001.
Web.
Lewis, Norman G, Davin, Laurence B, Dinkova-Kostova, Albena T, Fujita, Masayuki, Gang, David R, Sarkanen, Simo, & Ford, Joshua D. Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use. United States.
Lewis, Norman G, Davin, Laurence B, Dinkova-Kostova, Albena T, Fujita, Masayuki, Gang, David R, Sarkanen, Simo, and Ford, Joshua D. Mon .
"Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use". United States. https://www.osti.gov/servlets/purl/873645.
@article{osti_873645,
title = {Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use},
author = {Lewis, Norman G and Davin, Laurence B and Dinkova-Kostova, Albena T and Fujita, Masayuki and Gang, David R and Sarkanen, Simo and Ford, Joshua D},
abstractNote = {Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2001},
month = {1}
}
Works referenced in this record:
Stereospecificity of (+)-pinoresinol and (+)-lariciresinol reductases from Forsythia intermedia.
journal, December 1993
- Chu, A.; Dinkova, A.; Davin, L. B.
- Journal of Biological Chemistry, Vol. 268, Issue 36
Novel benzylic ether reductions in lignan biogenesis in Forsythia intermedia
journal, June 1993
- Katayama, Takeshi; Davin, Laurence B.; Chu, Alex
- Phytochemistry, Vol. 33, Issue 3
An extraordinary accumulation of (−)-pinoresinol in cell-free extracts of Forsythia intermedia: evidence for enantiospecific reduction of (+)-pinoresinol
journal, January 1992
- Katayama, Takeshi; Davin, Laurence B.; Lewis, Norman G.
- Phytochemistry, Vol. 31, Issue 11