Fluorescent labels and their use in separations

Mathies, Richard A; Glazer, Alexander; Ju, Jingyue

Fluorescent labels and their use in separations

Patent · Tue Dec 31 23:00:00 EST 1996

OSTI ID:871087

Mathies, Richard A ^[1]; Glazer, Alexander ^[2]; Ju, Jingyue ^[3]

El Cerrito, CA
Orinda, CA
Berkeley, CA

Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.

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Research Organization:: University of California

DOE Contract Number:: FG03-91ER61125

Assignee:: Regents of University of California (Oakland, CA)

Patent Number(s):: US 5654419

OSTI ID:: 871087

Country of Publication:: United States

Language:: English

References (16)

A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides Prober, J. M.; Trainor, G. L.; Dam, R. J. Science, Vol. 238, Issue 4825, p. 336-341 https://doi.org/10.1126/science.2443975	journal	October 1987
Energy transfer: a spectroscopic ruler. Stryer, L.; Haugland, R. P. Proceedings of the National Academy of Sciences, Vol. 58, Issue 2 https://doi.org/10.1073/pnas.58.2.719	journal	August 1967
Capillary array electrophoresis: an approach to high-speed, high-throughput DNA sequencing Mathies, Richard A.; Huang, Xiaohua C. Nature, Vol. 359, Issue 6391 https://doi.org/10.1038/359167a0	journal	September 1992
Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis Ansorge, Wilhelm; Sproat, Brian; Stegemann, Josef Nucleic Acids Research, Vol. 15, Issue 11 https://doi.org/10.1093/nar/15.11.4593	journal	January 1987
Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction Clegg, Robert M.; Murchie, Alastair I. H.; Zechel, Annelies Biochemistry, Vol. 31, Issue 20 https://doi.org/10.1021/bi00135a016	journal	May 1992
DNA sequencing using capillary array electrophoresis Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A. Analytical Chemistry, Vol. 64, Issue 18 https://doi.org/10.1021/ac00042a021	journal	September 1992
Design of Bifunctional Nucleic Acid Ligands Montenay-Garestier, T.; Sun, J. S.; Chomilier, J. The Jerusalem Symposia on Quantum Chemistry and Biochemistry https://doi.org/10.1007/978-94-011-3728-7_19	book	January 1990
Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus Middendorf, Lyle R.; Bruce, John C.; Bruce, Robert C. Electrophoresis, Vol. 13, Issue 1 https://doi.org/10.1002/elps.11501301103	journal	January 1992
DNA sequencing with chain-terminating inhibitors Sanger, F.; Nicklen, S.; Coulson, A. R. Proceedings of the National Academy of Sciences, Vol. 74, Issue 12 https://doi.org/10.1073/pnas.74.12.5463	journal	December 1977
Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer. Cardullo, R. A.; Agrawal, S.; Flores, C. Proceedings of the National Academy of Sciences, Vol. 85, Issue 23 https://doi.org/10.1073/pnas.85.23.8790	journal	December 1988
Fluorescence Energy Transfer as a Spectroscopic Ruler Stryer, L. Annual Review of Biochemistry, Vol. 47, Issue 1, p. 819-846 https://doi.org/10.1146/annurev.bi.47.070178.004131	journal	June 1978
Fluorescent dye phosphoramidite labelling of oligonucleotides Theisen, Pete; McCullum, Christie; Upadhya, Krishna Tetrahedron Letters, Vol. 33, Issue 35 https://doi.org/10.1016/S0040-4039(00)61181-4	journal	August 1992
End-to-end diffusion coefficients and distance distributions from fluorescence energy transfer measurements: Enhanced resolution by using multiple acceptors with different Förster distances Maliwal, Badri P.; Kuśba, Józef; Wiczk, Wieslaw Biophysical Chemistry, Vol. 46, Issue 3 https://doi.org/10.1016/0301-4622(93)80020-J	journal	May 1993
Fluorescence detection in automated DNA sequence analysis Smith, Lloyd M.; Sanders, Jane Z.; Kaiser, Robert J. Nature, Vol. 321, Issue 6071, p. 674-679 https://doi.org/10.1038/321674a0	journal	June 1986
The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis Smith, Lloyd M.; Fung, Steven; Hunkapiller, Michael W. Nucleic Acids Research, Vol. 13, Issue 7 https://doi.org/10.1093/nar/13.7.2399	journal	January 1985
Cognitive features of continuous antigenic determinants Geysen, H. Mario; Mason, Tom J.; Rodda, Stuart J. Journal of Molecular Recognition, Vol. 1, Issue 1 https://doi.org/10.1002/jmr.300010107	journal	February 1988

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Fluorescent labels and their use in separations

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References (16)

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