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Title: Fluorescent labels and their use in separations

Patent ·
OSTI ID:871087
 [1];  [2];  [3]
  1. El Cerrito, CA
  2. Orinda, CA
  3. Berkeley, CA
+ Show Author Affiliations

Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.

Research Organization:
Univ. of California (United States)
DOE Contract Number:
FG03-91ER61125
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5654419
OSTI ID:
871087
Country of Publication:
United States
Language:
English

References (16)

Fluorescence detection in automated DNA sequence analysis journal June 1986
Cognitive features of continuous antigenic determinants journal February 1988
End-to-end diffusion coefficients and distance distributions from fluorescence energy transfer measurements: Enhanced resolution by using multiple acceptors with different Förster distances journal May 1993
Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis journal January 1987
Fluorescent dye phosphoramidite labelling of oligonucleotides journal August 1992
Capillary array electrophoresis: an approach to high-speed, high-throughput DNA sequencing journal September 1992
DNA sequencing using capillary array electrophoresis journal September 1992
The synthesis of oligonucleotides containing an aliphatic amino group at the 5′ terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis journal January 1985
Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction journal May 1992
A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides journal October 1987
Design of Bifunctional Nucleic Acid Ligands book January 1990
Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus journal January 1992
DNA sequencing with chain-terminating inhibitors journal December 1977
Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer. journal December 1988
Energy transfer: a spectroscopic ruler. journal August 1967
Fluorescence Energy Transfer as a Spectroscopic Ruler journal June 1978

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Related Subjects

fluorescent
labels
separations
compositions
provided
comprising
sets
carrying
pairs
donor
acceptor
dye
molecules
designed
efficient
excitation
donors
single
wavelength
emission
wavelengths
donor-acceptor
modified
substantially
mobility
separation
conditions
varying
distance
pair
particularly
sequencing
nucleic
acids
nucleic acid
provided comprising
nucleic acids
dye molecules
separation conditions
fluorescent labels
single wavelength
comprising sets
fluorescent composition
acceptor pair
carrying pairs
acceptor dye
efficient excitation
labels carrying
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