Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

Patent · Tue Dec 31 23:00:00 EST 1985

OSTI ID:865829

Dolbeare, Frank A ^[1]; Gray, Joe W ^[1]

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1. Livermore, CA

A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• DOE Contract Number: W-7405-ENG-48
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4585736
• OSTI ID: 865829
• Country of Publication: United States
• Language: English

Related Subjects

/435/436/
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antibody
brdu
bromodeoxyuridine
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content
cycle
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dna
dna content
dna synthesis
flow
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halodeoxyuridine
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propidium
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single
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total
total dna
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