Flow cytometric measurement of total DNA and incorporated halodeoxyuridine
Patent
·
OSTI ID:5546303
A method is described for the simultaneous measurement of total cellular DNA content and of incorporated halodeoxyuridine which consists of: reacting cells with radioactive labeled halodeoxyuridine; denaturing cellular DNA with a denaturing agent; adding to the reaction medium monoclonal antibodies reactive with halodeoxyuridine; reacting the bound monoclonal antibodies with a labeled second antibody; incubating the mixture with a DNA stain; and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the halodeoxyuridine incorporated as a measure of DNA synthesis.
- Assignee:
- Dept. of Energy, Washington, DC
- Patent Number(s):
- US 4585736
- OSTI ID:
- 5546303
- Country of Publication:
- United States
- Language:
- English
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